KeyTec® BARK1, N-GST; C-StrepII
Size: Used for Developing BARK1 Biochemical Activity Assay or Binding Assay Models
Price: $ Inquire
CAT.: P1HI0162S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Please contact sales for details
This BARK1 recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating BARK1 protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0162S | KeyTec® BARK1, N-GST; C-StrepII | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
AA Sequences Uniprot: P25098, M1-L689(end)
Tag N-terminal GST tag, C-terminal Strep II tag
Molecular Weight 106.6 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.81 mg/mL by OD280
Purity >95% by SDS-PAGE
Form Liquid
Formulation 50M Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5
This BARK1 recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating BARK1 protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0162L | KeyTec® BARK1, N-GST; C-StrepII | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
English
