TR-FRET Assay Kit for Analysis of Biotherapeutics

The KeyTec® TR-FRET Human IgG detection kit is designed for quantitative measurement of human IgG in supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.

The KeyTec® TR-FRET Human Fc detection kit is designed for quantitative measurement of human IgG or human Fc-tagged protein in supernatant. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.

The KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.

The KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.

The KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.

The KeyTec® TR-FRET Hybridoma screening kit (His antigen, Rat IgG) is designed for the simple and rapid screening of positive rat hybridoma clones based on antigen-antibody affinity in cell supernatant.

KeyTec® TR-FRET Human FcRn Binding Kit is designed to screen IgGs and FcRn inhibitors that bind to Human FcRn, and provides the ideal solution to assess the half-life of different IgGs. This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec® TR-FRET Human CD16a (FcγRIIIA, 176V) Binding Kit is designed to screen IgGs that bind to Human CD16a (FcγRⅢA, 176V), and provides the ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD16a (FcγRIIIA, 176V) is hereinafter referred as CD16a (176V). This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec® TR-FRET Human CD16a (FcγRIIIA, 176F) Binding Kit is designed to screen IgGs that bind to Human CD16a (FcγRⅢA, 176F), and provides the ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD16a (FcγRIIIA, 176F) is hereinafter referred as CD16a (176F). This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. 

KeyTec® TR-FRET Human CD64 (FcγRI) Binding Kit is designed to screen IgGs that binds to Human CD64 (FcγRI), and provides an ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD64 (FcγRI) is hereinafter referred as CD64. This kit is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

This kit provides a homogeneous competitive TR-FRET assay for screening inhibitors of the TL1A–DR3 protein–protein interaction. Using a donor-labeled Streptavidin/TL1A complex and an acceptor-labeled DR3, the assay detects the binding signal at 665 nm. Compounds that inhibit the interaction reduce the signal in a concentration-dependent manner, enabling rapid and reliable screening of TL1A/DR3 blockers.

This kit offers a homogeneous competitive TR-FRET assay for screening inhibitors of the TL1A–DcR3 protein–protein interaction. Using a donor-labeled anti-Tag1 antibody to recognize Tag1-TL1A and an acceptor-labeled DcR3, the assay detects binding signals at 665 nm. Inhibitors that block the interaction reduce the signal in a concentration-dependent manner, enabling rapid and reliable screening of TL1A/DcR3 blockers.

This kit provides a homogeneous sandwich TR-FRET assay for rapid screening and affinity ranking of mouse hybridoma antibodies in culture supernatants. Using a donor-labeled anti-FLAG antibody to capture the FLAG-tagged antigen and an acceptor-labeled anti-mouse IgG antibody to detect the hybridoma-derived antibody, the assay measures proximity-dependent energy transfer signals at 665 nm, allowing quick and reliable identification of high-affinity positive clones.

This kit provides a homogeneous competitive TR-FRET assay for quantifying human IgG levels in various sample types. Using a donor-labeled anti-hFc antibody and an acceptor-labeled Human IgG tracer, the assay measures the competitive displacement signal at 665 nm, where the signal inversely correlates with the target concentration. It offers a rapid, accurate, and broad-range detection method for human IgG quantification.