The KeyTec® TR-FRET Kinase Series provides a highly efficient and precise solution for kinase activity detection. This product line includes kinase assay kits (such as protein kinase assay kit), specific substrates for kinases, tyrosine kinases (TKs), and cyclin-dependent kinases (CDKs), all leveraging the TR FRET assay kit technology platform.
The KeyTec® TR‑FRET Revamped TKs Kinase Assay Kit quantifies tyrosine kinase activity using a universal substrate or customized tyrosine‑specific substrates, compatible with >60 TKs. It employs a biotinylated peptide and streptavidin‑HX, along with a Solar Eu‑labeled anti‑phospho‑Tyr antibody, in a TR‑FRET sandwich format. The assay involves kinase reaction and detection steps, producing a signal proportional to phosphorylation. Simple, rapid, sensitive, and reproducible.
This kit measures serine/threonine kinase (STK) activity, validated for over 150 STKs. It supplies three biotinylated substrates (S1/S2/S3), streptavidin‑HX, and a phospho‑STK antibody labeled with Solar Eu, enabling quantification via phosphorylated substrate detection.
The KeyTec® TR‑FRET STK S1 Kinase Kit quantifies serine/threonine kinase activity, validated for >50 STKs. It supplies a biotinylated substrate, streptavidin‑HX, and a Solar Eu‑labeled phospho‑STK antibody. The TR‑FRET‑based sandwich assay enables simple, rapid, and sensitive quantification via phosphorylated substrate detection.
The KeyTec® TR‑FRET STK S2 Kinase Kit quantifies serine/threonine kinase activity, validated for >30 STKs. It supplies a biotinylated substrate, streptavidin‑HX, and a Solar Eu‑labeled phospho‑STK antibody. The TR‑FRET‑based sandwich assay enables simple, rapid, and sensitive quantification via phosphorylated substrate detection.
The KeyTec® TR‑FRET STK S3 Kinase Kit quantifies serine/threonine kinase activity, validated for >50 STKs. It supplies a biotinylated substrate, streptavidin‑HX, and a Solar Eu‑labeled phospho‑STK antibody. The TR‑FRET‑based sandwich assay enables simple, rapid, and sensitive quantification via phosphorylated substrate detection.
The KeyTec® TR‑FRET CDKs Kinase Kit quantifies CDK activity via phosphorylation of Rb S780. It includes a LA‑labeled substrate and a Solar Eu‑labeled phospho‑antibody. The TR‑FRET sandwich assay provides simple, rapid, and sensitive quantification.
The KeyTec® TR‑FRET CDKs Kinase Kit detects activity of CDK2 and CDK5, which phosphorylate ATF‑2 at T71. It supplies a LA‑labeled substrate and a Solar Eu‑labeled phospho‑antibody. The TR‑FRET sandwich assay involves kinase reaction (substrate phosphorylation) and detection (antibody binding to phosphorylated site), generating a signal proportional to activity. The assay is simple, rapid, sensitive, and reproducible.
KeyTec® TR-FRET TKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.
KeyTec® TR-FRET BTKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.
KeyTec® TR-FRET JAKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.
The KeyTec® TR‑FRET FGFRs substrate‑biotin kinase substrate can be used to develop kinase activity assays. When combined with the KeyTec® TR‑FRET TKs kinase kit (Cat. # A1080001L), it enables the construction of a TR‑FRET activity assay for evaluating relative enzyme activity or inhibitor screening.
The KeyTec® ActRIIA Kinase Kit detects ActRIIA activity by quantifying ADP produced from substrate phosphorylation. The assay includes a kinase reaction and an ADP detection step, enabling rapid kinase activity assessment
The KeyTec® ActRIIB Kinase Kit detects relative ActRIIB (ACVR2B) activity by quantifying ADP generated from a kinase–substrate reaction.
Our TR-FRET-based kinase activity kit delivers exceptional sensitivity and a wide dynamic range for precise quantification. The homogeneous, “mix-and-read” format of this kinase assay kit minimizes hands-on time and reduces assay artifacts, providing you with reliable data to accurately measure even subtle changes in kinase activity.
Accelerate your drug discovery pipeline with our optimized kinase activity assay kit. Designed for high-throughput applications, this ready-to-use system enables efficient IC50 determination and compound profiling. The robust performance of this protein kinase assay kit ensures high signal-to-background ratios, making it ideal for screening inhibitors against a broad panel of kinase targets.
Whether your focus is on STKs, CDKs, or TKs, our modular kinase assay kit platform offers tailored solutions. With both universal and family-specific substrates (e.g., for JAKs, BTKs, EGFRs), this versatile protein kinase assay kit supports research across diverse signaling pathways and target classes, all within a consistent and optimized assay workflow.
Maximize the reliability of your research outcomes with our rigorously validated kinase activity assay kit. The TR-FRET detection technology minimizes interference from compound autofluorescence or absorbance, ensuring superior assay stability and reproducibility run after run. Trust this robust kinase activity kit for consistent performance in demanding basic research and drug discovery applications.
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