KeyTec® ActRIIA kinase kit
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CAT.: A1080018S
The KeyTec® ActRIIA Kinase Assay Kit detects the relative activity level of ActRIIA (ACVR2A) kinase. The kit utilizes a reaction between the kinase and a specific substrate, and determines the relative kinase activity by quantifying the amount of ADP generated in the reaction.
The basic steps and principle of this kit are illustrated in the figure below. In the first phase (kinase reaction), the kinase phosphorylates the specific substrate in an optimized reaction buffer containing the kinase, specific substrate, and ATP, consuming ATP and generating ADP. In the second phase (detection), the level of ADP produced is quantified following the procedure provided with the ADP detection kit.
| CAT. | Description | Size |
| A1080018S | KeyTec® ActRIIA kinase kit | 500 tests |
|
• 1 vial 50 µg/vial ACVR2A • 1 vial 12.5 μL/vial Substrate • 1 vial 12.5 μL/vial ATP • 1 vial 2.5 μL/vial DTT • 1 vial 20 mL/vial Assay Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
The KeyTec® ActRIIA Kinase Assay Kit detects the relative activity level of ActRIIA (ACVR2A) kinase. The kit utilizes a reaction between the kinase and a specific substrate, and determines the relative kinase activity by quantifying the amount of ADP generated in the reaction.
The basic steps and principle of this kit are illustrated in the figure below. In the first phase (kinase reaction), the kinase phosphorylates the specific substrate in an optimized reaction buffer containing the kinase, specific substrate, and ATP, consuming ATP and generating ADP. In the second phase (detection), the level of ADP produced is quantified following the procedure provided with the ADP detection kit.
| CAT. | Description | Size |
| A1080018L | KeyTec® ActRIIA kinase kit | 5,000 tests |
|
• 1 vial 500 µg/vial ACVR2A • 1 vial 125 μL/vial Substrate • 1 vial 125 μL/vial ATP • 1 vial 25 μL/vial DTT • 1 bottle 200 mL/bottle Assay Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
The KeyTec® ActRIIA Kinase Assay Kit detects the relative activity level of ActRIIA (ACVR2A) kinase. The kit utilizes a reaction between the kinase and a specific substrate, and determines the relative kinase activity by quantifying the amount of ADP generated in the reaction.
The basic steps and principle of this kit are illustrated in the figure below. In the first phase (kinase reaction), the kinase phosphorylates the specific substrate in an optimized reaction buffer containing the kinase, specific substrate, and ATP, consuming ATP and generating ADP. In the second phase (detection), the level of ADP produced is quantified following the procedure provided with the ADP detection kit.
| CAT. | Description | Size |
| A1080018B | KeyTec® ActRIIA kinase kit | 50,000 tests |
|
• 10 vials 500 µg/vial ACVR2A • 10 vials 125 μL/vial Substrate • 10 vials 125 μL/vial ATP • 1 vial 250 μL/vial DTT • 2 bottles 1 L/bottle Assay Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |