KeyTec® TR-FRET Human TL1A/DR3 binding kit
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CAT.: A1040016D
The KeyTec® TR-FRET Human TL1A/DR3 Binding Assay Kit is designed for screening inhibitors that block the binding between TL1A and DR3. This kit utilizes KeyTec® TR-FRET technology in a competitive immunoassay format, offering advantages such as simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is illustrated below: The pre-formed complex of KeyTec® TR-FRET Solar Eu1-conjugated Streptavidin and Human TL1A protein can bind to KeyTec® TR-FRET LA2-conjugated Human DR3 protein, bringing Solar Eu (donor) and LA (acceptor) into close proximity and enabling energy resonance transfer upon external light excitation. By measuring the signal intensity at the specific wavelength (665 nm), the binding level between Human TL1A and Human DR3 can be determined. When the sample contains inhibitors that can disrupt the TL1A/DR3 interaction, they compete with DR3-LA for the binding sites on TL1A. As a result, the TR-FRET signal intensity is inversely proportional to the concentration of the inhibitor in the sample.
| CAT. | Description | Size |
| A1040016D | KeyTec® TR-FRET Human TL1A/DR3 binding kit | 100 tests |
|
• 1 × 1 vial (5 µL/vial) Biotin - TL1A & SA - Solar Eu Mix(100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET Human TL1A/DR3 Binding Assay Kit is designed for screening inhibitors that block the binding between TL1A and DR3. This kit utilizes KeyTec® TR-FRET technology in a competitive immunoassay format, offering advantages such as simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is illustrated below: The pre-formed complex of KeyTec® TR-FRET Solar Eu1-conjugated Streptavidin and Human TL1A protein can bind to KeyTec® TR-FRET LA2-conjugated Human DR3 protein, bringing Solar Eu (donor) and LA (acceptor) into close proximity and enabling energy resonance transfer upon external light excitation. By measuring the signal intensity at the specific wavelength (665 nm), the binding level between Human TL1A and Human DR3 can be determined. When the sample contains inhibitors that can disrupt the TL1A/DR3 interaction, they compete with DR3-LA for the binding sites on TL1A. As a result, the TR-FRET signal intensity is inversely proportional to the concentration of the inhibitor in the sample.
| CAT. | Description | Size |
| A1040016S | KeyTec® TR-FRET Human TL1A/DR3 binding kit | 500 tests |
|
• 1 × 1 vial (25 µL/vial) Biotin - TL1A & SA - Solar Eu Mix(100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET Human TL1A/DR3 Binding Assay Kit is designed for screening inhibitors that block the binding between TL1A and DR3. This kit utilizes KeyTec® TR-FRET technology in a competitive immunoassay format, offering advantages such as simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is illustrated below: The pre-formed complex of KeyTec® TR-FRET Solar Eu1-conjugated Streptavidin and Human TL1A protein can bind to KeyTec® TR-FRET LA2-conjugated Human DR3 protein, bringing Solar Eu (donor) and LA (acceptor) into close proximity and enabling energy resonance transfer upon external light excitation. By measuring the signal intensity at the specific wavelength (665 nm), the binding level between Human TL1A and Human DR3 can be determined. When the sample contains inhibitors that can disrupt the TL1A/DR3 interaction, they compete with DR3-LA for the binding sites on TL1A. As a result, the TR-FRET signal intensity is inversely proportional to the concentration of the inhibitor in the sample.
| CAT. | Description | Size |
| A1040016L | KeyTec® TR-FRET Human TL1A/DR3 binding kit | 5,000 tests |
|
• 1 × 1 vial (250 µL/vial) Biotin - TL1A & SA - Solar Eu Mix(100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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