KeyTec® TR-FRET Human Fc HiRange detection kit
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CAT.: A1040023S
The KeyTec® TR-FRET Human Fc Detection Kit is designed for the quantitative detection of human IgG levels in cell culture supernatants or purified samples. This kit utilizes KeyTec® TR-FRET technology in a competitive immunoassay format, offering advantages such as simplicity, speed, high accuracy, excellent reproducibility, and a wide detection range.
The basic principle of this method is illustrated below: The KeyTec® TR-FRET Solar Eu1-conjugated anti-Human Fc specific antibody and the KeyTec® TR-FRET LA2-conjugated Human IgG are used as detection reagents. When the mAb anti-hFc-Solar Eu binds to Human IgG-LA, the TR-FRET donor and acceptor are brought into close proximity, enabling energy resonance transfer upon external light excitation. Free Human IgG present in the sample competes with Human IgG-LA for binding to the mAb anti-hFc-Solar Eu. As a result, the signal intensity at the specific wavelength (665 nm) decreases with increasing concentrations of free Human IgG in the sample.
| CAT. | Description | Size |
| A1040023S | KeyTec® TR-FRET Human Fc HiRange detection kit | 1,000 tests |
|
• 1 vial（150 µL /vial）Human IgG Standard (15 mg/mL) • 1 bottle （50 mL/bottle） Biotherapeutics Diluent Buffer • 1 bottle （20 mL/bottle） Biotherapeutics Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET Human Fc Detection Kit is designed for the quantitative detection of human IgG levels in cell culture supernatants or purified samples. This kit utilizes KeyTec® TR-FRET technology in a competitive immunoassay format, offering advantages such as simplicity, speed, high accuracy, excellent reproducibility, and a wide detection range.
The basic principle of this method is illustrated below: The KeyTec® TR-FRET Solar Eu1-conjugated anti-Human Fc specific antibody and the KeyTec® TR-FRET LA2-conjugated Human IgG are used as detection reagents. When the mAb anti-hFc-Solar Eu binds to Human IgG-LA, the TR-FRET donor and acceptor are brought into close proximity, enabling energy resonance transfer upon external light excitation. Free Human IgG present in the sample competes with Human IgG-LA for binding to the mAb anti-hFc-Solar Eu. As a result, the signal intensity at the specific wavelength (665 nm) decreases with increasing concentrations of free Human IgG in the sample.
| CAT. | Description | Size |
| A1040023L | KeyTec® TR-FRET Human Fc HiRange detection kit | 10,000 tests |
|
• 2 vials（150 µL /vial）Human IgG Standard (15 mg/mL) • 1 bottle （200 mL/bottle） Biotherapeutics Diluent Buffer • 1 bottle （120 mL/bottle） Biotherapeutics Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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