KeyTec® ERN1, N-GST, C-Flag
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CAT.: P1HI0367S
AA Sequences Uniprot: O75460-1, M468-L977 (end)
Tag N-terminal GST tag, C-terminal Flag tag
Molecular Weight 85.7 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.74 mg/mL by OD280
Purity >80% by SDS-PAGE
Form Liquid
Formulation 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij 35, 10% glycerol, pH7.5
This ERN1 recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating ERN1 protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0367S | KeyTec® ERN1, N-GST, C-Flag | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
AA Sequences Uniprot: O75460-1, M468-L977 (end)
Tag N-terminal GST tag, C-terminal Flag tag
Molecular Weight 85.7 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.74 mg/mL by OD280
Purity >80% by SDS-PAGE
Form Liquid
Formulation 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij 35, 10% glycerol, pH7.5
This ERN1 recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating ERN1 protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0367L | KeyTec® ERN1, N-GST, C-Flag | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
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