KeyTec® cAMP TR-FRET KIT
Size: TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates
Price: $ Inquire
CAT.: A010101
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Used to detect cAMP , when Gs and Gi signaling pathways are activated or inhibited
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Used to detect cAMP, when Gs and Gi signaling pathways are activated or inhibited
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Used to detect cAMP , when Gs and Gi signaling pathways are activated or inhibited
KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
| CAT. | Description | Size |
| A010101 | KeyTec® cAMP TR-FRET KIT | 1,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 5 nmoles/vial cAMP Standard Lyophilized, A015011 • 1 × 50 μL/vial cAMP tracer (100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
| CAT. | Description | Size |
| A010012 | KeyTec® cAMP TR-FRET KIT | 10,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 2 × 5 nmoles/vial cAMP Standard Lyophilized, A015011 • 1 × 500 μL/vial cAMP tracer (100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
| CAT. | Description | Size |
| A010014 | KeyTec® cAMP TR-FRET KIT | 100,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 6 × 5 nmoles/vial cAMP Standard Lyophilized, A015011 • 1 × 5mL/vial cAMP tracer (100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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