KeyTec® cAMP TR-FRET KIT

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TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Used to detect cAMP , when Gs and Gi signaling pathways are activated or inhibited

TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Used to detect cAMP, when Gs and Gi signaling pathways are activated or inhibited

TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Used to detect cAMP , when Gs and Gi signaling pathways are activated or inhibited

Size:

1,000 tests

10,000 tests

100,000 tests

Price:

$ Inquire

CAT.:

A010101

Delivery:

Available in Stock

Price:

$ Inquire

CAT.:

A010012

Delivery:

Available in Stock

Price:

$ Inquire

CAT.:

A010014

Delivery:

Available in Stock

Sample Request PDF Download

Overview

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.

Performance

Standard curve

Protocols

/uploads/file/20260109/protocol_a010101_a010012_a010014_keytec® camp tr-fret kit.pdf Download

Components

CAT.

Description

Size

A010101

KeyTec® cAMP TR-FRET KIT

1,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 50 μL/vial mAb anti-cAMP - LA(100X)

• 1 × 5 nmoles/vial cAMP Standard Lyophilized, A015011

• 1 × 50 μL/vial cAMP tracer (100X)
• 1 × 2 mL/bottle Detection Buffer (5X)

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-60℃ or below

Overview

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.

Performance

Standard curve

Protocols

/uploads/file/20260109/protocol_a010101_a010012_a010014_keytec® camp tr-fret kit.pdf Download

Components

CAT.

Description

Size

A010012

KeyTec® cAMP TR-FRET KIT

10,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 500 μL/vial mAb anti-cAMP - LA(100X)

• 2 × 5 nmoles/vial cAMP Standard Lyophilized, A015011

• 1 × 500 μL/vial cAMP tracer (100X)
• 1 × 20 mL/bottle Detection Buffer (5X)

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-60℃ or below

Overview

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.

Performance

Standard curve

Protocols

/uploads/file/20260109/protocol_a010101_a010012_a010014_keytec® camp tr-fret kit.pdf Download

Components

CAT.

Description

Size

A010014

KeyTec® cAMP TR-FRET KIT

100,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 5mL/vial mAb anti-cAMP - LA(100X)

• 6 × 5 nmoles/vial cAMP Standard Lyophilized, A015011

• 1 × 5mL/vial cAMP tracer (100X)
• 1 × 200 mL/bottle Detection Buffer (5X)

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-60℃ or below

FAQs

Q

What is the principle of a TR-FRET cAMP assay?

A

This cAMP FRET assay uses a competitive TR-FRET immunoassay. A cAMP tracer (donor) and an anti-cAMP antibody (acceptor) bind to each other, producing a 665 nm signal. Free cAMP from cell lysates competes with the tracer, causing the signal to decrease as cAMP concentration increases.

Q

Does this kit measure intracellular or extracellular cAMP?

A

It measures intracellular cAMP. Cells are lysed to release free cAMP into the lysate, and the assay detects total cAMP inside the cells at the time of lysis.

Q

Can I use this kit for Gi-coupled receptors that decrease cAMP? How should I set controls?

A

Yes. Add Forskolin to raise baseline cAMP. Key controls: Forskolin only (baseline), Forskolin + Gi agonist (to see cAMP reduction), untreated cells (basal level), and a Gi antagonist (to validate the window). Optimize Forskolin and cell density so signals fall within the standard curve’s linear range.

KeyTec® cAMP TR-FRET KIT

Overview

Performance

Protocols

Components

Notices

Overview

Performance

Protocols

Components

Notices

Overview

Performance

Protocols

Components

Notices

FAQs

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