KeyTec® FLT3-ITD, N-GST; C-His
Size: Used for Developing FLT3-ITD Biochemical Activity Assay or Binding Assay Models
Price: $ Inquire
CAT.: P1HI0146S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
AA Sequences Uniprot: P36888-1，R571-S993
Tag N-terminal GST tag, C-terminal 6x His tag
Molecular Weight 77.8kDa
Species Human
Expression Host Sf9
Protein Concentration 0.42mg/mL by OD280
Purity > 95% by SDS-PAGE
Form Liquid
Formulation 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij35, pH7.5
The FLT3-ITD activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
| CAT. | Description | Size |
| P1HI0146S | KeyTec® FLT3-ITD, N-GST; C-His | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
AA Sequences Uniprot: P36888-1，R571-S993
Tag N-terminal GST tag, C-terminal 6x His tag
Molecular Weight 77.8kDa
Species Human
Expression Host Sf9
Protein Concentration 0.42mg/mL by OD280
Purity > 95% by SDS-PAGE
Form Liquid
Formulation 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij35, pH7.5
The FLT3-ITD activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
| CAT. | Description | Size |
| P1HI0146L | KeyTec® FLT3-ITD, N-GST; C-His | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
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