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KeyTec® FLT3-ITD, N-GST; C-His

Quick replication of product information

KeyTec® FLT3-ITD, N-GST; C-His

Size: Used for Developing FLT3-ITD Biochemical Activity Assay or Binding Assay Models

Price: $ Inquire

CAT.: P1HI0146S

激酶_1.1
激酶_1.2
激酶_1.1
激酶_1.1
激酶_1.1
激酶_1.2
激酶_1.1
激酶_1.1
Used for Developing FLT3-ITD Biochemical Activity Assay or Binding Assay Models

•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing FLT3-ITD Biochemical Activity Assay or Binding Assay Models

•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ Inquire
CAT.:
P1HI0146S
Delivery:
Available in Stock
Price:
$ Inquire
CAT.:
P1HI0146L
Delivery:
Available in Stock
Sample Request PDF Download
KeyTec® FLT3-ITD, N-GST; C-His
10 μg
10 μg
100 μg
CAT.: P1HI0146S
$ Inquire $ Inquire
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® FLT3-ITD, N-GST; C-His recombinant protein with N-terminal GST tag + 3C cleavage site and C-terminal 6xHis tag was purified by Ni-NTA affinity chromatography. The FLT3-ITD protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: P36888-1, R571-S993
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 77.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.24 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5

Performance

P1HI0146_2.1
P1HI0146_2.2

The FLT3-ITD activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

KeyTec® FLT3-ITD, N-GST; C-His P1HI0146S
KeyTec® FLT3-ITD, N-GST; C-His P1HI0146S

Protocols

/uploads/file/20260126/coa_p1hi0146_abw01sa.pdf
/uploads/file/20260126/coa_p1hi0146_abw01sa.pdf Download

Components

CAT. Description Size
P1HI0146S KeyTec® FLT3-ITD, N-GST; C-His 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® FLT3-ITD, N-GST; C-His recombinant protein with N-terminal GST tag + 3C cleavage site and C-terminal 6xHis tag was purified by Ni-NTA affinity chromatography. The FLT3-ITD protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: P36888-1, R571-S993
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 77.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.24 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5

Performance

P1HI0146_2.1
P1HI0146_2.2

The FLT3-ITD activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

146 1
CTL

Protocols

/uploads/file/20260126/coa_p1hi0146_abw01sa.pdf
/uploads/file/20260126/coa_p1hi0146_abw01sa.pdf Download

Components

CAT. Description Size
P1HI0146L KeyTec® FLT3-ITD, N-GST; C-His 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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