KeyTec® TR-FRET PD1/PD-L1 Binding Assay kit

KeyTec® TR-FRET PD1/PD-L1 Binding Assay Kit is designed for the quantitative measurement of the interaction between human PD1 and PD-L1 proteins. This kit provides a Tag1-PD-L1 protein, a Tag2-PD1 protein, an anti-tag1 antibody labeled with KeyTec® TR-FRET Solar Eu*1 (mAb anti-Tag1 - Solar Eu) ,an anti-tag2 antibody labeled with KeyTec® TR-FRET LA*2 (mAb anti-Tag2 - LA) and other detection reagents. These components enable the high-throughput screening of small molecule inhibitors, peptides, or antibody blockers.

This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1:

The binding of the mAb anti-Tag1 - Solar Eu, the Tag1-PD-L1 protein, the mAb anti-Tag2 - LA and the Tag2-PD1 protein brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. Compounds, peptides, or antibodies that block the PD1/PD-L1 interaction will cause a decrease in the TR-FRET signal.

*1  KeyTec® TR-FRET Solar Eu: TR-FRET Donor

*2  KeyTec® TR-FRET HX: TR-FRET Acceptor