KeyTec® ARAF[Y301D/Y302D], N-GST
Size: Used for Developing ARAF[Y301D/Y302D] Biochemical Activity Assay or Binding Assay Models
Price: $ 660
CAT.: P1HI0103S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
AA Sequences Uniprot: P10398-1; K282-P606(end), Y301D/Y302D
Tag N-terminal GST tag
Molecular Weight 63.3 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.55 mg/mL by OD280
Purity >40% by SDS-PAGE
Form Liquid
Formulation 20 mM Hepes, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
The ARAF[Y301D/Y302D] activity was detected using ADP-Glo technology. The reaction was performed by incubating the ARAF[Y301D/Y302D] protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
| CAT. | Description | Size |
| P1HI0103S | KeyTec® ARAF[Y301D/Y302D], N-GST | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
AA Sequences Uniprot: P10398-1; K282-P606(end), Y301D/Y302D
Tag N-terminal GST tag
Molecular Weight 63.3 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.55 mg/mL by OD280
Purity >40% by SDS-PAGE
Form Liquid
Formulation 20 mM Hepes, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
The ARAF[Y301D/Y302D] activity was detected using ADP-Glo technology. The reaction was performed by incubating the ARAF[Y301D/Y302D] protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
| CAT. | Description | Size |
| P1HI0103L | KeyTec® ARAF[Y301D/Y302D], N-GST | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
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