KeyTec® BTK[C481S], N-GST
Size: Used for Developing BTK[C481S] Biochemical Activity Assay or Binding Assay Models
Price: $ 945
CAT.: P1HI0008S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
KeyTec® BTK[C481S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the BTK[C481S] protein was dimer in the solution. The BTK[C481S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q06187, A2-S659, C481S
Tag：N-terminal GST tag
Molecular Weight：101.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.41 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：Liquid
Formulation：50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH 7.5
The BTK[C481S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[C481S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
| CAT. | Description | Size |
| P1HI0008S | KeyTec® BTK[C481S], N-GST | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
| CAT. | Description | Size |
| P1HI0008L | KeyTec® BTK[C481S], N-GST | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
English
