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KeyTec® BTK[C481S], N-GST

Quick replication of product information

KeyTec® BTK[C481S], N-GST

Size: Used for Developing BTK[C481S] Biochemical Activity Assay or Binding Assay Models

Price: $ 945

CAT.: P1HI0008S

激酶_1.1
激酶_1.2
激酶_1.1
激酶_1.1
激酶_1.1.jpg
激酶_1.2.jpg
激酶_1.1.jpg
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Used for Developing BTK[C481S] Biochemical Activity Assay or Binding Assay Models

•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing BTK[C481S] Biochemical Activity Assay or Binding Assay Models

•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 945
CAT.:
P1HI0008S
Delivery:
2-3 weeks
Price:
$ 5291
CAT.:
P1HI0008L
Delivery:
2-3 weeks
Sample Request PDF Download
KeyTec® BTK[C481S], N-GST
10 μg
10 μg
100 μg
CAT.: P1HI0008S
$ 945 $ 5291
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® BTK[C481S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the BTK[C481S] protein was dimer in the solution. The BTK[C481S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q06187, A2-S659, C481S
Tag：N-terminal GST tag
Molecular Weight：101.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.41 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：Liquid
Formulation：50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH 7.5

Performance

P1HI0008_2.1
P1HI0008_2.2

The BTK[C481S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[C481S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

P1HI0008_2.3
P1HI0008_2.4

Protocols

/uploads/file/product/COA_P1HI0008_K130320011_EN.pdf
/uploads/file/product/COA_P1HI0008_K130320011_EN.pdf Download

Components

CAT. Description Size
P1HI0008S KeyTec® BTK[C481S], N-GST 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® BTK[C481S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the BTK[C481S] protein was dimer in the solution. The BTK[C481S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q06187, A2-S659, C481S
Tag：N-terminal GST tag
Molecular Weight：101.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.41 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：Liquid
Formulation：50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH 7.5

Performance

P1HI0008_2.1.png
P1HI0008_2.2.png
The BTK[C481S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[C481S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0008_2.3.png
P1HI0008_2.4.png

Protocols

/uploads/file/product/COA_P1HI0008_K130320011_EN.pdf
/uploads/file/product/COA_P1HI0008_K130320011_EN.pdf Download

Components

CAT. Description Size
P1HI0008L KeyTec® BTK[C481S], N-GST 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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