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KeyTec® Enhanced Luciferase Detection Kit

Quick replication of product information

KeyTec® Enhanced Luciferase Detection Kit

Size: Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

Price: $ 113

CAT.: A2000100N

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Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and robust luminescence signal
• The luminescence signal is not only high but also robust, with a half-life of approximately 1 h. It is suitable for most assay formats

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and robust luminescence signal
• The luminescence signal is not only high but also robust, with a half-life of approximately 1 h. It is suitable for most assay formats

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and robust luminescence signal
• The luminescence signal is not only high but also robust, with a half-life of approximately 1 h. It is suitable for most assay formats

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and robust luminescence signal
• The luminescence signal is not only high but also robust, with a half-life of approximately 1 h. It is suitable for most assay formats

Size:
10 mL
2*50 mL
2*250 mL
4*250 mL
Price:
$ 113
CAT.:
A2000100N
Delivery:
Available in Stock
Price:
$ 705
CAT.:
A2000101N
Delivery:
Available in Stock
Price:
$ 2340
CAT.:
A2000102N
Delivery:
Available in Stock
Price:
$ 5380
CAT.:
A2000103N
Delivery:
Available in Stock
KeyTec® Enhanced Luciferase Detection Kit
10 mL
10 mL
2*50 mL
2*250 mL
4*250 mL
CAT.: A2000100N
$ 113 $ 705 $ 2340 $ 5380
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® Enhanced luciferase Detection kit is designed for the sensitive and robust detection of firefly luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with firefly luciferase released by cells, generating a sensitive and robust luminescence signal. This process provides a highly sensitive, robust, and homogeneous assay for the detection of firefly luciferase reporter gene expression in mammalian cells.
EN_Firefly luciferase_2.png

Performance

Use KeyTec® Enhanced Luciferase Detection Kit to detect the overexpression of firefly luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in.“Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
A2000100N_3.png

Protocols

/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf
/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT. Description Size
A2000100N KeyTec® Enhanced Luciferase Detection Kit 10 mL
Each kit contains sufficient reagents to perform 100 tests of 100 μl/well.
The kit contains the following components:
• 1 × 200 μL KeyTec® Enhanced Luciferase Substrate 50X
• 1 × 10 mL KeyTec® Enhanced Luciferase Cell lysis buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -40 ℃

-40 ℃

Overview

KeyTec® Enhanced luciferase Detection kit is designed for the sensitive and robust detection of firefly luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with firefly luciferase released by cells, generating a sensitive and robust luminescence signal. This process provides a highly sensitive, robust, and homogeneous assay for the detection of firefly luciferase reporter gene expression in mammalian cells.
EN_Firefly luciferase_2.png

Performance

Use KeyTec® Enhanced Luciferase Detection Kit to detect the overexpression of firefly luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in.“Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
A2000100N_3.png

Protocols

/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf
/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT. Description Size
A2000101N KeyTec® Enhanced Luciferase Detection Kit 2*50 mL
Each kit contains sufficient reagents to perform 1,000 tests of 100 μl/well.
The kit contains the following components:
• 2 × 1 mL KeyTec® Enhanced Luciferase Substrate 50X
• 2 × 50 mL KeyTec® Enhanced Luciferase Cell lysis buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -40 ℃

-40 ℃

Overview

KeyTec® Enhanced luciferase Detection kit is designed for the sensitive and robust detection of firefly luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with firefly luciferase released by cells, generating a sensitive and robust luminescence signal. This process provides a highly sensitive, robust, and homogeneous assay for the detection of firefly luciferase reporter gene expression in mammalian cells.
EN_Firefly luciferase_2.png

Performance

Use KeyTec® Enhanced Luciferase Detection Kit to detect the overexpression of firefly luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in.“Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
A2000100N_3.png

Protocols

/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf
/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT. Description Size
A2000102N KeyTec® Enhanced Luciferase Detection Kit 2*250 mL
Each kit contains sufficient reagents to perform 5,000 tests of 100 μl/well.
The kit contains the following components:
• 5 × 2 mL KeyTec® Enhanced Luciferase Substrate 50X
• 2 × 250 mL KeyTec® Enhanced Luciferase Cell lysis buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -40 ℃

-40 ℃

Overview

KeyTec® Enhanced luciferase Detection kit is designed for the sensitive and robust detection of firefly luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with firefly luciferase released by cells, generating a sensitive and robust luminescence signal. This process provides a highly sensitive, robust, and homogeneous assay for the detection of firefly luciferase reporter gene expression in mammalian cells.
EN_Firefly luciferase_2.png

Performance

Use KeyTec® Enhanced Luciferase Detection Kit to detect the overexpression of firefly luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in.“Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
A2000100N_3.png

Protocols

/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf
/uploads/file/product/Protocol_A2000100N_A2000101N_A2000102N_A2000103N_KeyTec® Enhanced Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT. Description Size
A2000103N KeyTec® Enhanced Luciferase Detection Kit 4*250 mL
Each kit contains sufficient reagents to perform 10,000 tests of 100 μl/well.
The kit contains the following components:
• 2 × A2000102N KeyTec® Enhanced Luciferase Detection Kit (5,000 tests)

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -40 ℃

-40 ℃

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