KeyTec® Stable Luciferase Detection Kit (no DTT)
Size: Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays
Price: $ 82
CAT.: A2000700N
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Long half-life: Stable luminescence signal with 2-hour half-life and without DTT
• With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Long half-life: Stable luminescence signal with 2-hour half-life and without DTT
• With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Long half-life: Stable luminescence signal with 2-hour half-life and without DTT
• With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
KeyTec® Stable Luciferase Detection Kit (no DTT) is designed for the exceedingly robust detection of Firefly luciferase reporter gene assays without Dithiothreitol, suitable for High Throughput Screening (HTS). Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a long half-life luminescence signal. The process provides a high repeatability, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.
Use KeyTec® Stable Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
| CAT. | Description | Size |
| A2000700N | KeyTec® Stable Luciferase Detection Kit (no DTT) | 10 mL |
|
Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-40 ℃ |
KeyTec® Stable Luciferase Detection Kit (no DTT) is designed for the exceedingly robust detection of Firefly luciferase reporter gene assays without Dithiothreitol, suitable for High Throughput Screening (HTS). Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a long half-life luminescence signal. The process provides a high repeatability, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.
Use KeyTec® Stable Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
| CAT. | Description | Size |
| A2000701N | KeyTec® Stable Luciferase Detection Kit (no DTT) | 2*50 mL |
|
Each kit contains sufficient reagents to perform 1,000 tests of 100 μL/well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-40 ℃ |
KeyTec® Stable Luciferase Detection Kit (no DTT) is designed for the exceedingly robust detection of Firefly luciferase reporter gene assays without Dithiothreitol, suitable for High Throughput Screening (HTS). Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a long half-life luminescence signal. The process provides a high repeatability, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.
Use KeyTec® Stable Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
| CAT. | Description | Size |
| A2000702N | KeyTec® Stable Luciferase Detection Kit (no DTT) | 2*250 mL |
|
Each kit contains sufficient reagents to perform 5,000 tests of 100 μL/well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-40 ℃ |
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