KeyTec® Stable Luciferase Detection Kit (no DTT)
Size: Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays
Price: $ 82
CAT.: A2000700N
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Long half-life: Stable luminescence signal with 2-hour half-life and without DTT
• With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Long half-life: Stable luminescence signal with 2-hour half-life and without DTT
• With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Long half-life: Stable luminescence signal with 2-hour half-life and without DTT
• With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
Use KeyTec® Stable Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
| CAT. | Description | Size |
| A2000700N | KeyTec® Stable Luciferase Detection Kit (no DTT) | 10 mL |
|
Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-40 ℃ |
Use KeyTec® Stable Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
| CAT. | Description | Size |
| A2000701N | KeyTec® Stable Luciferase Detection Kit (no DTT) | 2*50 mL |
|
Each kit contains sufficient reagents to perform 1,000 tests of 100 μL/well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-40 ℃ |
Use KeyTec® Stable Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
| CAT. | Description | Size |
| A2000702N | KeyTec® Stable Luciferase Detection Kit (no DTT) | 2*250 mL |
|
Each kit contains sufficient reagents to perform 5,000 tests of 100 μL/well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-40 ℃ |
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