KeyTec® TR-FRET Human CD64 (FcγRI) binding kit
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CAT.: A1040015S
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High S/B: The kit provides a superior window
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High S/B: The kit provides a superior window
KeyTec® TR-FRET Human CD64 (FcγRI) Binding Kit is designed to screen IgGs that binds to Human CD64 (FcγRI), and provides an ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD64 (FcγRI) is hereinafter referred as CD64. This kit is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle is outlined in Figure 1.
KeyTec® TR-FRET Solar Eu*1- anti-Tag1 antibody can specifically recognize the CD64 protein with Tag1. The KeyTec® TR-FRET LA*2- Human IgG can bind to the CD64 protein, bringing Solar Eu (referred as donor) and LA (referred as acceptor) close to each other. Under excitation by an external light source, energy resonance transfer occurs between the donor and the acceptor. The binding affinity between CD64 and Human IgG can be determined by detecting the signal intensity at a specific wavelength (665 nm). Free Human IgGs or Human Fc-tagged proteins in the sample can compete with Human IgG-LA for the binding sites on CD64, and the TR-FRET signal intensity is inversely proportional to the free Human IgGs or Human Fc-tagged proteins in the sample.
*¹ KeyTec® TR-FRET LA: TR-FRET Acceptor
*² KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1040015S | KeyTec® TR-FRET Human CD64 (FcγRI) binding kit | 500 tests |
|
• 1 × 25 µL/vial mAb anti-Tag1 - Solar Eu(100X) • 1 × 25 µL/vial Human IgG - LA (100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET Human CD64 (FcγRI) Binding Kit is designed to screen IgGs that binds to Human CD64 (FcγRI), and provides an ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD64 (FcγRI) is hereinafter referred as CD64. This kit is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle is outlined in Figure 1.
KeyTec® TR-FRET Solar Eu*1- anti-Tag1 antibody can specifically recognize the CD64 protein with Tag1. The KeyTec® TR-FRET LA*2- Human IgG can bind to the CD64 protein, bringing Solar Eu (referred as donor) and LA (referred as acceptor) close to each other. Under excitation by an external light source, energy resonance transfer occurs between the donor and the acceptor. The binding affinity between CD64 and Human IgG can be determined by detecting the signal intensity at a specific wavelength (665 nm). Free Human IgGs or Human Fc-tagged proteins in the sample can compete with Human IgG-LA for the binding sites on CD64, and the TR-FRET signal intensity is inversely proportional to the free Human IgGs or Human Fc-tagged proteins in the sample.
*¹ KeyTec® TR-FRET LA: TR-FRET Acceptor
*² KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1040015L | KeyTec® TR-FRET Human CD64 (FcγRI) binding kit | 5,000 tests |
|
• 1 × 250 µL/vial mAb anti-Tag1 - Solar Eu(100X) • 1 × 250 µL/vial Human IgG - LA (100X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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