KeyTec® TR-FRET Phospho-EGFR (Y1068) Kit
Size: Sandwich Model TR-FRET Assay for Detecting of P-EGFR (Y1068) in Cell Lysates
Price: $
CAT.: A1060121D
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding P-EGFR (Y1068), ensuring results accuracy
• Wider range for high concentration, making it more suitable for agonist or antagonistic (with agonist pre-treatment)
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding P-EGFR (Y1068), ensuring results accuracy
• Wider range for high concentration, making it more suitable for agonist or antagonistic (with agonist pre-treatment)
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding P-EGFR (Y1068), ensuring results accuracy
• Wider range for high concentration, making it more suitable for agonist or antagonistic (with agonist pre-treatment)
KeyTec® TR-FRET Phospho-EGFR (Y1068) Kit can detect the level of intracellular phosphorylated EGFR (Y1068) protein after cell lysis. Epidermal Growth Factor Receptor (EGFR, also known as ErbB-1 or HER1) is a member of the ErbB family of receptor tyrosine kinases. It is located on the cell membrane surface and, upon ligand binding, induces dimerization, which leads to the activation of its intrinsic kinase domain and phosphorylation at specific tyrosine residues. This triggers receptor autophosphorylation and activates downstream signaling pathways, including MAPK, AKT, and JNK pathways, ultimately promoting cell proliferation. EGFR is closely associated with tumor cell proliferation, angiogenesis, tumor invasion and metastasis, and inhibition of apoptosis, making it a critically important therapeutic target in oncology.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are conjugated to KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, respectively: one antibody specifically recognizes the phosphorylation site, while the other recognizes another epitope of the target protein. When the two antibodies simultaneously bind to distinct epitopes of the phosphorylated EGFR (Y1068) protein, the TR-FRET donor and acceptor are brought into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. The level of phosphorylated EGFR (Y1068) protein can then be determined by measuring the signal intensity at a specific wavelength (665 nm).
In A431 cells, stimulate phosphorylation of EGFR protein at the Y1068 site
In A431 cells, inhibit phosphorylation of EGFR protein at the Y1068 site
| CAT. | Description | Size |
| A1060121D | KeyTec® TR-FRET Phospho-EGFR (Y1068) Kit | 100 tests |
|
• 1 vial 5 µL/vial Phospho-INSR β (Y1150/Y1151) - Solar Eu antibody • 1 vial 5 µL/vial Phospho-INSR β (Y1150/Y1151) - LA antibody • 1 vial 20 µL/vial Positive control lysate • 1 vial 50 µL/vial Phosphatase Inhibitor Cocktail (100X) • 1 vial 1 mL/vial Phosphoproteins Lysis Buffer D (5X) • 1 vial 500 µL/vial Phosphoproteins Detection Buffer (1X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET Phospho-EGFR (Y1068) Kit can detect the level of intracellular phosphorylated EGFR (Y1068) protein after cell lysis. Epidermal Growth Factor Receptor (EGFR, also known as ErbB-1 or HER1) is a member of the ErbB family of receptor tyrosine kinases. It is located on the cell membrane surface and, upon ligand binding, induces dimerization, which leads to the activation of its intrinsic kinase domain and phosphorylation at specific tyrosine residues. This triggers receptor autophosphorylation and activates downstream signaling pathways, including MAPK, AKT, and JNK pathways, ultimately promoting cell proliferation. EGFR is closely associated with tumor cell proliferation, angiogenesis, tumor invasion and metastasis, and inhibition of apoptosis, making it a critically important therapeutic target in oncology.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are conjugated to KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, respectively: one antibody specifically recognizes the phosphorylation site, while the other recognizes another epitope of the target protein. When the two antibodies simultaneously bind to distinct epitopes of the phosphorylated EGFR (Y1068) protein, the TR-FRET donor and acceptor are brought into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. The level of phosphorylated EGFR (Y1068) protein can then be determined by measuring the signal intensity at a specific wavelength (665 nm).
In A431 cells, stimulate phosphorylation of EGFR protein at the Y1068 site
In A431 cells, inhibit phosphorylation of EGFR protein at the Y1068 site
| CAT. | Description | Size |
| A1060121S | KeyTec® TR-FRET Phospho-EGFR (Y1068) Kit | 500 tests |
|
• 1 vial 25 µL/vial Phospho-EGFR (Y1068) - Solar Eu antibody • 1 vial 25 µL/vial Phospho-EGFR (Y1068) - LA antibody • 1 vial 100 µL/vial Positive control lysate • 1 vial 400 µL/vial Phosphatase Inhibitor Cocktail (100X) • 1 bottle 8 mL/bottle Phosphoproteins Lysis Buffer A(5X) • 1 bottle 2.5 mL/bottle Phosphoproteins Detection Buffer (1X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET Phospho-EGFR (Y1068) Kit can detect the level of intracellular phosphorylated EGFR (Y1068) protein after cell lysis. Epidermal Growth Factor Receptor (EGFR, also known as ErbB-1 or HER1) is a member of the ErbB family of receptor tyrosine kinases. It is located on the cell membrane surface and, upon ligand binding, induces dimerization, which leads to the activation of its intrinsic kinase domain and phosphorylation at specific tyrosine residues. This triggers receptor autophosphorylation and activates downstream signaling pathways, including MAPK, AKT, and JNK pathways, ultimately promoting cell proliferation. EGFR is closely associated with tumor cell proliferation, angiogenesis, tumor invasion and metastasis, and inhibition of apoptosis, making it a critically important therapeutic target in oncology.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are conjugated to KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, respectively: one antibody specifically recognizes the phosphorylation site, while the other recognizes another epitope of the target protein. When the two antibodies simultaneously bind to distinct epitopes of the phosphorylated EGFR (Y1068) protein, the TR-FRET donor and acceptor are brought into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. The level of phosphorylated EGFR (Y1068) protein can then be determined by measuring the signal intensity at a specific wavelength (665 nm).
In A431 cells, stimulate phosphorylation of EGFR protein at the Y1068 site
In A431 cells, inhibit phosphorylation of EGFR protein at the Y1068 site
| CAT. | Description | Size |
| A1060121L | KeyTec® TR-FRET Phospho-EGFR (Y1068) Kit | 5,000 tests |
|
• 1 vial 250 µL/vial Phospho-EGFR (Y1068) - Solar Eu antibody • 1 vial 250 µL/vial Phospho-EGFR (Y1068) - LA antibody • 2 vials 100 µL/vial Positive control lysate • 1 bottle 3 mL/bottle Phosphatase Inhibitor Cocktail (100X) • 1 bottle 60 mL/bottle Phosphoproteins Lysis Buffer A(5X) • 1 bottle 25 mL/bottle Phosphoproteins Detection Buffer (1X) |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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