KeyTec® TR-FRET Total STAT6 Kit
Size: Sandwich Model TR-FRET Assay for Detecting of STAT6 in Cell Lysates
Price: $
CAT.: A1060092D
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding STAT6, ensuring results accuracy
• Wider range for high concentration, making it more suitable for agonist or antagonistic (with agonist pre-treatment)
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding STAT6, ensuring results accuracy
• Wider range for high concentration, making it more suitable for agonist or antagonistic (with agonist pre-treatment)
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding STAT6, ensuring results accuracy
• Wider range for high concentration, making it more suitable for agonist or antagonistic (with agonist pre-treatment)
KeyTec® TR-FRET Total STAT6 Kit can be used to measure the expression level of intracellular STAT6 total protein in cell lysates. This kit is a sandwich immunoassay developed using KeyTec® TR‑FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below: a pair of specific antibodies are separately conjugated to the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to distinct epitopes of the STAT6 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The expression level of STAT6 total protein is determined by measuring the signal intensity at a specific wavelength (665 nm).
In HeLa cells, stimulate phosphorylation of STAT6 protein at the Y641 site
In HeLa cells, inhibit phosphorylation of STAT6 protein at the Y641 site
| CAT. | Description | Size |
| A1060092D | KeyTec® TR-FRET Total STAT6 Kit | 100 tests |
|
• 1 vial 5 µL/vial mAb anti-total-STAT6 - Solar Eu • 1 vial 20 µL/vial mAb anti-total-STAT6 - LA • 1 vial 40 µL/vial Positive control lysate • 1 vial 50 µL/vial Phosphatase Inhibitor Cocktail • 1 vial 1 mL/vial Phosphoproteins Lysis Buffer B • 1 vial 50 µL/vial Phosphoproteins Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET Total STAT6 Kit can be used to measure the expression level of intracellular STAT6 total protein in cell lysates. This kit is a sandwich immunoassay developed using KeyTec® TR‑FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below: a pair of specific antibodies are separately conjugated to the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to distinct epitopes of the STAT6 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The expression level of STAT6 total protein is determined by measuring the signal intensity at a specific wavelength (665 nm).
In HeLa cells, stimulate phosphorylation of STAT6 protein at the Y641 site
In HeLa cells, inhibit phosphorylation of STAT6 protein at the Y641 site
| CAT. | Description | Size |
| A1060092S | KeyTec® TR-FRET Total STAT6 Kit | 500 tests |
|
• 1 vial 25 µL/vial mAb anti-total-STAT6 - Solar Eu • 1 vial 100 µL/vial mAb anti-total-STAT6 - LA • 1 vial 100 µL/vial Positive control lysate • 1 vial 250 µL/vial Phosphatase Inhibitor Cocktail • 1 bottle 5 mL/bottle Phosphoproteins Lysis Buffer B • 1 vial 250 µL/vial Phosphoproteins Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET Total STAT6 Kit can be used to measure the expression level of intracellular STAT6 total protein in cell lysates. This kit is a sandwich immunoassay developed using KeyTec® TR‑FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below: a pair of specific antibodies are separately conjugated to the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to distinct epitopes of the STAT6 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The expression level of STAT6 total protein is determined by measuring the signal intensity at a specific wavelength (665 nm).
In HeLa cells, stimulate phosphorylation of STAT6 protein at the Y641 site
In HeLa cells, inhibit phosphorylation of STAT6 protein at the Y641 site
| CAT. | Description | Size |
| A1060092L | KeyTec® TR-FRET Total STAT6 Kit | 5,000 tests |
|
• 1 vial 250 µL/vial mAb anti-total-STAT6 - Solar Eu • 1 vial 1 mL/vial mAb anti-total-STAT6 - LA • 2 vials 100 µL/vial Positive control lysate • 1 bottle 2.5 mL/bottle Phosphatase Inhibitor Cocktail • 1 bottle 50 mL/bottle Phosphoproteins Lysis Buffer B • 1 bottle 2.5 mL/bottle Phosphoproteins Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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