KeyTec® TR-FRET Streptavidin-Bright Tb
Size: TR-FRET Assay for Binding Biotinylated Proteins in PPI Assay
Price: $ Inquire
CAT.: A1020065S
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding biotinylated proteins, ensuring results accuracy
• Compatible with proteins from most brands for PPI assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Specificity: Use specific antibodies for binding biotinylated proteins, ensuring results accuracy
• Compatible with proteins from most brands for PPI assays
The KeyTec® TR-FRET Bright Tb1-conjugated Streptavidin is designed for use in TR-FRET assay development. Taking a protein-protein interaction detection model as an example: when the interaction between Biotinylated Protein A and His-tagged Protein B needs to be detected, the KeyTec® TR-FRET Bright Tb1-conjugated Streptavidin and the KeyTec® TR-FRET Acceptor*2-conjugated anti-His tag antibody are brought into proximity due to the protein-protein interaction. Upon excitation by an external light source, energy resonance transfer (FRET) occurs between the donor and acceptor, and specific signals can be read at 665 nm or 520 nm. The intensity of this specific signal is proportional to the extent of the protein-protein interaction. This type of assay model can be used for screening inhibitors or blockers (compounds, antibodies, etc.) of protein-protein interactions.
| CAT. | Description | Size |
| A1020065S | KeyTec® TR-FRET Streptavidin-Bright Tb | 1,000 tests |
|
• 1 × 1 vial（10 pmoles） KeyTec® TR-FRET Streptavidin-Bright Tb, Lyophilized |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
The KeyTec® TR-FRET Bright Tb1-conjugated Streptavidin is designed for use in TR-FRET assay development. Taking a protein-protein interaction detection model as an example: when the interaction between Biotinylated Protein A and His-tagged Protein B needs to be detected, the KeyTec® TR-FRET Bright Tb1-conjugated Streptavidin and the KeyTec® TR-FRET Acceptor*2-conjugated anti-His tag antibody are brought into proximity due to the protein-protein interaction. Upon excitation by an external light source, energy resonance transfer (FRET) occurs between the donor and acceptor, and specific signals can be read at 665 nm or 520 nm. The intensity of this specific signal is proportional to the extent of the protein-protein interaction. This type of assay model can be used for screening inhibitors or blockers (compounds, antibodies, etc.) of protein-protein interactions.
| CAT. | Description | Size |
| A1020065L | KeyTec® TR-FRET Streptavidin-Bright Tb | 10,000 tests |
|
• 1 × 1 vial（100 pmoles） KeyTec® TR-FRET Streptavidin-Bright Tb, Lyophilized |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
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