KeyTec® TR-FRET TKs kinase kit
Size: TR-FRET Assay for KINASE ACTIVITY Measurement of TKs
Price: $ Inquire
CAT.: A1080001S
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• TK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• TK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• TK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
KeyTec® TR-FRET TKs kinase kit is designed for measuring the Tyrosine kinase (TKs) activity. Tyrosine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 60 tyrosine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho Tyr-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody and Streptavidin-HX containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylated substrate. This binding brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET HX: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080001S | KeyTec® TR-FRET TKs kinase kit | 1,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
KeyTec® TR-FRET TKs kinase kit is designed for measuring the Tyrosine kinase (TKs) activity. Tyrosine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 60 tyrosine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho Tyr-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody and Streptavidin-HX containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylated substrate. This binding brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET HX: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080001L | KeyTec® TR-FRET TKs kinase kit | 20,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
KeyTec® TR-FRET TKs kinase kit is designed for measuring the Tyrosine kinase (TKs) activity. Tyrosine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 60 tyrosine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho Tyr-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody and Streptavidin-HX containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylated substrate. This binding brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET HX: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080001B | KeyTec® TR-FRET TKs kinase kit | 100,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
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