KeyTec® TR-FRET Human FcRn binding kit
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CAT.: A1040012S
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High S/B: The kit provides a superior window
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High S/B: The kit provides a superior window
KeyTec® TR-FRET Human FcRn Binding Kit is designed to screen IgGs and FcRn inhibitors that bind to Human FcRn, and provides the ideal solution to assess the half-life of different IgGs. This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle is outlined in Figure 1.
The ready-to-use pre-mix of KeyTec® TR-FRET Solar Eu¹- Streptavidin and Biotinylated Human FcRn protein can bind to KeyTec® TR-FRET LA²- Human IgG, bringing Solar Eu (referred as donor) and LA (referred as acceptor) close to each other. Under excitation by an external light source, resonance energy transfer occurs between the donor and the acceptor. The binding affinity between FcRn and Human IgG can be determined by detecting the signal intensity at a specific wavelength (665 nm). Free Human IgGs or Human Fc-tagged proteins in the sample can compete with Human IgG-LA for the binding sites on FcRn. The TR-FRET signal intensity is inversely proportional to the concentration of free Human IgGs or Human Fc-tagged proteins in the sample.
*¹ KeyTec® TR-FRET LA: TR-FRET Acceptor
*² KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1040012S | KeyTec® TR-FRET Human FcRn binding kit | 500 tests |
|
• 1 × 25 µL/vial Biotin-FcRn & SA-Solar Eu Mix (100X) • 1 × 25 µL/vial Human IgG - LA (100X) • 1 ×30 mL/bottle A1010044S FcRn Detection Buffer |
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| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET Human FcRn Binding Kit is designed to screen IgGs and FcRn inhibitors that bind to Human FcRn, and provides the ideal solution to assess the half-life of different IgGs. This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle is outlined in Figure 1.
The ready-to-use pre-mix of KeyTec® TR-FRET Solar Eu¹- Streptavidin and Biotinylated Human FcRn protein can bind to KeyTec® TR-FRET LA²- Human IgG, bringing Solar Eu (referred as donor) and LA (referred as acceptor) close to each other. Under excitation by an external light source, resonance energy transfer occurs between the donor and the acceptor. The binding affinity between FcRn and Human IgG can be determined by detecting the signal intensity at a specific wavelength (665 nm). Free Human IgGs or Human Fc-tagged proteins in the sample can compete with Human IgG-LA for the binding sites on FcRn. The TR-FRET signal intensity is inversely proportional to the concentration of free Human IgGs or Human Fc-tagged proteins in the sample.
*¹ KeyTec® TR-FRET LA: TR-FRET Acceptor
*² KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1040012L | KeyTec® TR-FRET Human FcRn binding kit | 5,000 tests |
|
• 1 × 250 µL/vial Biotin-FcRn & SA-Solar Eu Mix (100X) • 1 × 250 µL/vial Human IgG - LA (100X) • 1 ×120 mL/bottle A1010044L FcRn Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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