KeyTec® TR-FRET Human IL8 Detection kit
Size: TR-FRET Assay for Quantitative Measurement of Human IL8 Protein in Supernatant
Price: $ Inquire
CAT.: A1070004S
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 89 pg/mL to 10,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 89 pg/mL to 10,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
KeyTec® TR-FRET Human IL8 Detection Kit is designed for the quantitative determination of Human IL8 in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
The principle, outlined in Figure 1: Two specific antibodies are conjugated to KeyTec® TR-FRET Solar Eu*1 (Donor) and KeyTec® TR-FRET LA*2 (Acceptor), respectively. When the two antibodies bind to different epitopes of Human IL8 protein, the TR-FRET Donor and Acceptor are brought into close proximity. Excitation of the Donor with an external light source (laser or flash lamp) triggers Fluorescence Resonance Energy Transfer (FRET) to the Acceptor. The expression level of Human IL8 protein is proportional to the signal intensity at a specific wavelength (665 nm).
*1 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
*2 KeyTec® TR-FRET LA: TR-FRET Acceptor
| CAT. | Description | Size |
| A1070004S | KeyTec® TR-FRET Human IL8 Detection kit | 1,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 1,000 tests/vial mAb anti-IL8 - LA(Lyophilized) • 2 vials A1070004N Human IL8 Standard(Lyophilized) • 1 × 20 mL/bottle Cytokine Diluent Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
KeyTec® TR-FRET Human IL8 Detection Kit is designed for the quantitative determination of Human IL8 in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
The principle, outlined in Figure 1: Two specific antibodies are conjugated to KeyTec® TR-FRET Solar Eu*1 (Donor) and KeyTec® TR-FRET LA*2 (Acceptor), respectively. When the two antibodies bind to different epitopes of Human IL8 protein, the TR-FRET Donor and Acceptor are brought into close proximity. Excitation of the Donor with an external light source (laser or flash lamp) triggers Fluorescence Resonance Energy Transfer (FRET) to the Acceptor. The expression level of Human IL8 protein is proportional to the signal intensity at a specific wavelength (665 nm).
*1 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
*2 KeyTec® TR-FRET LA: TR-FRET Acceptor
| CAT. | Description | Size |
| A1070004L | KeyTec® TR-FRET Human IL8 Detection kit | 10,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 10,000 tests/vial mAb anti-IL8 - LA(Lyophilized) • 2 vials A1070004N Human IL8 Standard(Lyophilized) • 1 × 50 mL/bottle Cytokine Diluent Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
English
