KeyTec® TR-FRET Human IL8 Detection kit
Size: TR-FRET Assay for Quantitative Measurement of Human IL8 Protein in Supernatant
Price: $ Inquire
CAT.: A1070004D
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 89 pg/mL to 10,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 89 pg/mL to 10,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 89 pg/mL to 10,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
KeyTec® TR-FRET Human IL8 Detection Kit can be used to measure the expression level of Human IL8 in samples such as cell culture supernatants. This kit is a sandwich immunoassay developed based on KeyTec® TR-FRET technology, featuring simplicity, rapidity, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are separately conjugated with the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to different epitopes of the Human IL8 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The level of Human IL8 protein can be determined by detecting the signal intensity at a specific wavelength (665 nm).
标准曲线结果展示
不同基质溶液测试结果
| CAT. | Description | Size |
| A1070004D | KeyTec® TR-FRET Human IL8 Detection kit | 100 tests |
|
• 1 vial 5 µL/vial mAb anti-IL8- Solar Eu • 1 vial 5 µL/vial mAb anti-IL8- LA • 1 vial 50 µL/vial Human IL8 Standard • 2 vials 2 mL/vial Cytokine Diluent Buffer • 1 vial 2 mL/vial Cytokine Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
KeyTec® TR-FRET Human IL8 Detection Kit can be used to measure the expression level of Human IL8 in samples such as cell culture supernatants. This kit is a sandwich immunoassay developed based on KeyTec® TR-FRET technology, featuring simplicity, rapidity, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are separately conjugated with the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to different epitopes of the Human IL8 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The level of Human IL8 protein can be determined by detecting the signal intensity at a specific wavelength (665 nm).
标准曲线结果展示
不同基质溶液测试结果
| CAT. | Description | Size |
| A1070004S | KeyTec® TR-FRET Human IL8 Detection kit | 1,000 tests |
|
• 1 vial 50 µL/vial mAb anti-IL8- Solar Eu • 1 vial 50 µL/vial mAb anti-IL8- LA • 2 vials (Lyophilized) Human IL8 Standard • 1 bottle 20 mL/bottle Cytokine Diluent Buffer • 1 bottle 10 mL/bottle Cytokine Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
KeyTec® TR-FRET Human IL8 Detection Kit can be used to measure the expression level of Human IL8 in samples such as cell culture supernatants. This kit is a sandwich immunoassay developed based on KeyTec® TR-FRET technology, featuring simplicity, rapidity, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are separately conjugated with the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to different epitopes of the Human IL8 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The level of Human IL8 protein can be determined by detecting the signal intensity at a specific wavelength (665 nm).
标准曲线结果展示
不同基质溶液测试结果
| CAT. | Description | Size |
| A1070004L | KeyTec® TR-FRET Human IL8 Detection kit | 10,000 tests |
|
• 1 vial 500 µL/vial mAb anti-IL8- Solar Eu • 1 vial 500 µL/vial mAb anti-IL8- LA • 2 vials (Lyophilized) Human IL8 Standard • 1 bottle 50 mL/bottle Cytokine Diluent Buffer • 1 bottle 50 mL/bottle Cytokine Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
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