KeyTec® TR-FRET Human IL4 Detection kit
Size: TR-FRET Assay for Quantitative Measurement of Human IL4 Protein in Supernatant
Price: $ Inquire
CAT.: A1070013D
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 47.7 pg/mL to 5,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 47.7 pg/mL to 5,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 47.7 pg/mL to 5,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
KeyTec® TR-FRET Human IL4 Detection Kit can be used to measure the expression level of Human IL4 in samples such as cell culture supernatants. This kit is a sandwich immunoassay developed based on KeyTec® TR-FRET technology, featuring simplicity, rapidity, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are separately conjugated with the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to different epitopes of the Human IL4 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The level of Human IL4 protein can be determined by detecting the signal intensity at a specific wavelength (665 nm).
Standard curve
Results of testing in different matrix solutions
| CAT. | Description | Size |
| A1070013D | KeyTec® TR-FRET Human IL4 Detection kit | 100 tests |
|
• 1 vial mAb anti-IL4- Solar Eu（400X) • 1 vial mAb anti-IL4- LA (400X) • 1 vial 50 ng/mL Human IL4 Standard • 2 vials 2 mL/vial A1010006D Cytokine Diluent Buffer • 1 vial 2 mL/vial A1010007D Cytokine Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
KeyTec® TR-FRET Human IL4 Detection Kit can be used to measure the expression level of Human IL4 in samples such as cell culture supernatants. This kit is a sandwich immunoassay developed based on KeyTec® TR-FRET technology, featuring simplicity, rapidity, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are separately conjugated with the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to different epitopes of the Human IL4 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The level of Human IL4 protein can be determined by detecting the signal intensity at a specific wavelength (665 nm).
Standard curve
Results of testing in different matrix solutions
| CAT. | Description | Size |
| A1070013S | KeyTec® TR-FRET Human IL4 Detection kit | 1,000 tests |
|
• 1 vial (50 µL/vial) mAb anti-IL4- Solar Eu（400X) • 1 vial (50 µL/vial) mAb anti-IL4- LA (400X) • 2 vials A1070013N Human IL4 Standard • 1 bottle 20 mL/bottle A1010006S Cytokine Diluent Buffer • 1 bottle 10 mL/bottle A1010007S Cytokine Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
KeyTec® TR-FRET Human IL4 Detection Kit can be used to measure the expression level of Human IL4 in samples such as cell culture supernatants. This kit is a sandwich immunoassay developed based on KeyTec® TR-FRET technology, featuring simplicity, rapidity, high specificity, high sensitivity, and good reproducibility.
The basic principle of this method is illustrated in the figure below. A pair of specific antibodies are separately conjugated with the fluorescence energy donor Solar Eu and the fluorescence energy acceptor LA. When the two antibodies bind to different epitopes of the Human IL4 protein, energy resonance transfer occurs between the donor and acceptor upon excitation by an external light source. The level of Human IL4 protein can be determined by detecting the signal intensity at a specific wavelength (665 nm).
Standard curve
Results of testing in different matrix solutions
| CAT. | Description | Size |
| A1070013L | KeyTec® TR-FRET Human IL4 Detection kit | 10,000 tests |
|
• 1 vial (500 µL/vial) mAb anti-IL4- Solar Eu（400X) • 1 vial (500 µL/vial) mAb anti-IL4- LA (400X) • 2 vials A1070013N Human IL4 Standard • 1 bottle 50 mL/bottle A1010006L Cytokine Diluent Buffer • 1 bottle 50 mL/bottle A1010007L Cytokine Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
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