KeyTec® TR-FRET STK S1 kinase kit
Size: TR-FRET Assay for KINASE ACTIVITY Measurement of STKs-s1 subtrate
Price: $ Inquire
CAT.: A1080003S
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• STK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• STK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• STK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
KeyTec® TR-FRET STK S1 kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 50 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody and Streptavidin-HX containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylated substrate. This binding brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET HX: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080003S | KeyTec® TR-FRET STK S1 kinase kit | 1,000 tests |
|
• 1 vial （1,000 tests/vial） p-STK mAb - Solar Eu Lyophilized |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
KeyTec® TR-FRET STK S1 kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 50 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody and Streptavidin-HX containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylated substrate. This binding brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET HX: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080003L | KeyTec® TR-FRET STK S1 kinase kit | 20,000 tests |
|
• 1 vial （20,000 tests/vial） p-STK mAb - Solar Eu Lyophilized |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
KeyTec® TR-FRET STK S1 kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 50 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody and Streptavidin-HX containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylated substrate. This binding brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET HX: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080003B | KeyTec® TR-FRET STK S1 kinase kit | 100,000 tests |
|
• 5 vials （20,000 tests/vial） p-STK mAb - Solar Eu Lyophilized |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
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