VKEY-BIO provides comprehensive biological assays and bioassay testing solutions designed to meet the rigorous demands of high-throughput drug screening. Our comprehensive product portfolio includes a cell Viability Detection Kit, Reporter Gene Detection Kit, In Vivo Imaging Reagent, PPI Tool-Box Reagent, Biomarker Detection Kit, Hybridoma Screening Kit, Antibodies Concentration Detection Kit, Phosphoprotein Detection Kit, Kinase and Epigenetic Enzyme solutions, Premium Consumables. Beyond our extensive catalog of ready-to-use solutions, we offer customized assay development services to address your unique research requirements. At VKEY-BIO, we combine scientific excellence with practical innovation to drive your discoveries forward.
VKEY-BIO's homogeneous biological assays feature a simple "add-incubate-read" workflow that cuts processing time and steps while ensuring accurate results. Our streamlined format reduces errors and boosts efficiency.
VKEY-BIO offers high-performance bioassay testing solutions at competitive prices, delivering optimal results through advanced biological assay methods while staying within budget.
VKEY-BIO guarantees precision in every product batch of our biological assays through uncompromising quality control, delivering reliable bioassay testing solutions that ensure consistent, reproducible results.
Before initiating a TR-FRET assay, it's essential to verify that your microplate reader is equipped with a TRF module. If you're uncertain about your reader's compatibility, please send the reader type and brand to technical-support@vkeybio.com for further assistance.
The most common routes of administration for D-Luciferin potassium salt in small animal live imaging are intraperitoneal (IP) injection and tail vein injection. Each route has its own characteristics:
Intraperitoneal injection: Provides slow diffusion and onset of luminescence, reaching peak signal in approximately 10-20 minutes, but offers a longer duration of luminescence.
Tail vein injection: Offers faster diffusion and onset of luminescence, reaching peak signal in about 2-5 minutes, but provides a shorter duration of luminescence.
This ratio ensures that each of the four identical peptide chains of Streptavidin (SA) can bind to one biotin molecule. It's crucial to match the concentrations of SA-LA/HX and Europium (Eu) to avoid the "hook effect" caused by excessive Streptavidin, which could compromise the accuracy of the assay.
The Stimulation buffer is designed to resuspend cells and maintain their normal cellular activity within a short period (within 2 hours). When preparing it, it's important to add IBMX to a final concentration of 0.5 mM. It's recommended to prepare the Stimulation buffer immediately before use to ensure its effectiveness.
In a PPI model, the Binding Assay Diluent Buffer is primarily used for diluting protein samples, while the Solar Eu/Tb Detection Buffer is specifically optimized for detecting antibody dilutions.
Yes, the Cell Viability Assay Kit can be used to detect the viability of both adherent and suspension cells. The principle of the assay involves quantitatively measuring the ATP content of lysed cells to determine the number of metabolically active living cells
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