Bioluminescence and fluorescence are key for in vivo optical imaging. Bioluminescence relies on firefly luciferase, expressed from a reporter gene, reacting with an injected substrate (e.g., KeyTec® D-Luciferin potassium salt) to produce light.
This substrate is highly efficient, soluble, and stable, enabling strong luminescence in both live-cell and animal studies.
KeyTec® D-LUCIFERIN POTASSIUM SALT is designed for imaging living Firefly luciferase reporter gene cells in vitro or in vivo. It is a highly efficient, stable and reliable substrate for Firefly luciferase, producing a sensitive and robust luminescence signal. Its excellent water and fat solubility properties allow it to easily cross cell membranes.
The purity can reach more than 99%.
Maximum solubility in water up to 42 mg/mL.
It can penetrate cell membranes to provide the substrate required for luciferase reaction.
High-performance at competitive prices.
KeyTec® D - luciferin potassium salt penetrates the cell membrane and enters living cells, where it reacts with firefly luciferase to generate oxyluciferin and a luminescent phenomenon. Signals can be detected using a microplate reader or a chemiluminescence imaging instrument.
In vivo imaging technology can be used to monitor biological processes such as tumor growth and metastasis, inflammation, specific gene expression and drug efficacy in living animals.
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