KeyTec® CDK1/CyclinE2, N-GST/N-GST
Size: Used for Developing CDK1/CyclinE2 Biochemical Activity Assay or Binding Assay Models
Price: $ 661
CAT.: P1HI0180S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
AA Sequences Uniprot: CDK1: P06493-1; M1-M297(end); CyclinE2: O96020, M1-H404
Tag CDK1: N-terminal GST tag; CyclinE2: N-terminal GST tag
Molecular Weight CDK1: 60.7 kDa; CyclinE2: 72.6 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.32 mg/mL by OD280
Purity >95% by SDS-PAGE
Form Liquid
Formulation 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5
This CDK1/CyclinE2 recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating CDK1/CyclinE2 protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0180S | KeyTec® CDK1/CyclinE2, N-GST/N-GST | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
AA Sequences Uniprot: CDK1: P06493-1; M1-M297(end); CyclinE2: O96020, M1-H404
Tag CDK1: N-terminal GST tag; CyclinE2: N-terminal GST tag
Molecular Weight CDK1: 60.7 kDa; CyclinE2: 72.6 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.32 mg/mL by OD280
Purity >95% by SDS-PAGE
Form Liquid
Formulation 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5
This CDK1/CyclinE2 recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating CDK1/CyclinE2 protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0180L | KeyTec® CDK1/CyclinE2, N-GST/N-GST | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
English
