KeyTec® TR-FRET CDKs kinase kit - ATF2(T71) substrate
Size: TR-FRET Assay for KINASE ACTIVITY Measurement of CDKs - ATF2(T71) SUBSTRATE
Price: $ Inquire
CAT.: A1080007S
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• CDK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• CDK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• CDK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels
KeyTec® TR-FRET CDKs kinase detection kit is designed for measuring the cyclin-dependent kinases (CDKs) activity. The protien kinase family , which comprises multiple members such as CDK2 and 5, is known to phosphorylate Threonine 71 (T71) of Activating transcription factor 2 (ATF-2). The assay kit provides a CDKs-specific substrate labeled with KeyTec® TR-FRET LA*1 , a phospho-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylation site. This binding brings a TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET LA: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080007S | KeyTec® TR-FRET CDKs kinase kit - ATF2(T71) substrate | 1,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
KeyTec® TR-FRET CDKs kinase detection kit is designed for measuring the cyclin-dependent kinases (CDKs) activity. The protien kinase family , which comprises multiple members such as CDK2 and 5, is known to phosphorylate Threonine 71 (T71) of Activating transcription factor 2 (ATF-2). The assay kit provides a CDKs-specific substrate labeled with KeyTec® TR-FRET LA*1 , a phospho-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylation site. This binding brings a TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET LA: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080007L | KeyTec® TR-FRET CDKs kinase kit - ATF2(T71) substrate | 20,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
KeyTec® TR-FRET CDKs kinase detection kit is designed for measuring the cyclin-dependent kinases (CDKs) activity. The protien kinase family , which comprises multiple members such as CDK2 and 5, is known to phosphorylate Threonine 71 (T71) of Activating transcription factor 2 (ATF-2). The assay kit provides a CDKs-specific substrate labeled with KeyTec® TR-FRET LA*1 , a phospho-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:
Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.
TR-FRET Detection: Adding an antibody containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylation site. This binding brings a TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.
*1 KeyTec® TR-FRET LA: TR-FRET Acceptor
*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor
| CAT. | Description | Size |
| A1080007B | KeyTec® TR-FRET CDKs kinase kit - ATF2(T71) substrate | 100,000 tests |
|
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
2-8 ℃ |
English
