KeyTec® TR-FRET mAb anti-HA-Green

The KeyTec® TR-FRET Green2-conjugated Mouse anti-HA tag antibody (Mouse mAb anti-HA tag) is designed for use in TR-FRET assay development. Taking a protein-protein interaction detection model as an example: when the interaction between His-tagged Protein A and HA-tagged Protein B needs to be detected, the KeyTec® TR-FRET Solar Tb1-conjugated anti-His tag antibody and the KeyTec® TR-FRET Green*2-conjugated anti-HA tag antibody are brought into proximity due to the protein-protein interaction. Upon excitation by an external light source, energy resonance transfer (FRET) occurs between the donor and acceptor, and specific signals can be read at 520 nm. The intensity of this specific signal is proportional to the extent of the protein-protein interaction. This type of assay model can be used for screening inhibitors or blockers (compounds, antibodies, etc.) of protein-protein interactions.