KeyTec® TR-FRET STK discovery kinase kit

Name: KeyTec® TR-FRET STK discovery kinase kit
Brand: Shanghai VKEY Biotechnologies Co.,Ltd.

Quick replication of product information

TR-FRET Assay for KINASE ACTIVITY Measurement of STKs

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• STK Activity Measurement via Phosphorylated Substrate Detection
• Flexible Profiling: From Individual Kinases to Full Kinase Panels

Size:

1,000 tests

Price:

$ Inquire

CAT.:

A1080002S

Delivery:

Available in Stock

KeyTec® TR-FRET STK discovery kinase kit

1,000 tests

CAT.: A1080002S

$ Inquire

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® TR-FRET STK discovery kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 150 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides three types of biotinylated substrates(S1/S2/S3), Streptavidin-HX*1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrates.

This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. The principle, outlined in Figure 1, involves two main phases:

Kinase Reaction: The specific substrate is phosphorylated by the target kinase in the optimized reaction buffer with ATP.

TR-FRET Detection: Adding an antibody and Streptavidin-HX containing EDTA (to terminate the kinase reaction) that specifically binds to the phosphorylated substrate. This binding brings the TR-FRET donor and acceptor into close proximity, enabling resonance energy transfer (RET) upon excitation and generating a TR-FRET signal. The intensity of TR-FRET signal is directly proportional to the level of phosphorylated substrate.

*1 KeyTec® TR-FRET HX: TR-FRET Acceptor

*2 KeyTec® TR-FRET Solar Eu: TR-FRET Donor

KeyTec® TR-FRET STK discovery kinase kit A1080002S

Performance

Protocols

protocol_a1080002s_keytec®_tr-fret_stk_discovery_kinase_kit Download

Components

CAT.	Description	Size
A1080002S	KeyTec® TR-FRET STK discovery kinase kit	1,000 tests
• 1 vial p-STK mAb-Solar Eu (Lyophilized) • 2 vials Streptavidin- HX (Lyophilized) • 1 vial STK Substrate 1- biotin (Lyophilized) • 1 vial STK Substrate 2- biotin (Lyophilized) • 1 vial STK Substrate 3- biotin (Lyophilized) • 1 vial DTT (Lyophilized) • 1 vial MgCl2 (1 M) • 1 vial CaCl2 (1 M) • 1 bottle Kinase Enzymatic buffer (5X) • 1 bottle Kinase Detection buffer (含 EDTA)