KeyTec® WRN, N-His; C-Flag
Size: Used for Developing WRN Biochemical Activity Assay or Binding Assay Models
Price: $ 915
CAT.: P4HI0033S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
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This WRN recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating WRN protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P4HI0033S | KeyTec® WRN, N-His; C-Flag | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
AA Sequences Uniprot: Q14191, M1-S1432 (end)
Tag N-terminal 6x His tag, C-terminal Flag tag
Molecular Weight 165.6 kDa
Species Human
Expression Host Sf9
Protein Concentration 0.43 mg/mL by OD280
Purity > 60% by SDS-PAGE
Form Liquid
Formulation 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
This WRN recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating WRN protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P4HI0033L | KeyTec® WRN, N-His; C-Flag | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
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