Proteases are a class of enzymes that catalyze the hydrolysis of proteins or peptides by cleaving peptide bonds, breaking down target molecules into smaller peptides or free amino acids.
Proteases play critical roles in numerous biological processes and diseases, including fetal and postnatal development, reproduction, signal transduction, immune responses, autoimmune disorders, degenerative diseases, and cancer—where precise detection of protease activity is essential. Our high-quality recombinant proteases are ideal tools for protease activity assay development, enabling accurate measurement of enzyme kinetics, inhibitor screening, and mechanistic studies in life science research.
A strict QC (quality control) test report is provided for each batch.
Each batch has been verified for enzyme activity.
Verified by common homogeneous methods such as TR-FRET, Luminescence and fluorescence.
High-performance at competitive prices.
For KeyTec® protein products, each batch of protein is subjected to a series of rigorous validation procedures to obtain data such as purity, monomer/polymer morphology, enzyme activity information, positive compound validation information, and so forth. When evaluating protease-related products, an enzyme assay for protease is an essential part of the validation process, ensuring accurate assessment of enzymatic activity and product reliability.
Our product line includes human, mouse, and rat MASP-2 (CCP1/CCP2/SP) protease domains, Tryptase, HRV-3C protease, along with heat shock proteins (HSP90α, HSP90β) and transcription factor HSF1. Each protein is expressed in E. coli or insect cells and purified to high purity, verified by SDS-PAGE, ensuring reliable performance in enzyme assay for protease studies, protein interaction analysis, and tag removal applications.
The MASP-2 protease domains (human/mouse/rat), Tryptase, and HRV-3C protease are ideal tools for establishing robust protease activity assay systems. Researchers can utilize these active enzymes for kinetic characterization, substrate profiling, and inhibitor screening in drug discovery workflows, with multiple species variants available for translational studies.
With N-terminal His-Ubiquitin, C-terminal His, N-terminal His-Avi, and N-terminal GST tag options, our proteins offer versatility for various detection methods. Whether developing a protease assay, studying protein-protein interactions, or removing affinity tags from recombinant proteins, these products provide consistent quality and batch-to-batch reproducibility for life science research.
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