Time-Resolved Fluorescence (TRF) Analysis: This method is based on the long fluorescence effect of lanthanide chelates and involves fluorescence analysis from both time and fluorescence intensity dimensions. Since its establishment in the 1980s, this technique has effectively eliminated the interference of non-specific fluorescence, significantly enhancing analytical sensitivity.
1.Superior Signal-to-Noise Ratio:The solution efficiently dissociates Eu³⁺/Sm³⁺ from solid-phase bound conjugates and forms highly fluorescent micellar chelates. By eliminating short-lived background fluorescence, it ensures that only long-lived lanthanide signals are captured, delivering exceptional sensitivity for your time resolved fluorescence assay.
2.Wide Dynamic Range and High Accuracy:Optimized to release and enhance lanthanide ions, this solution enables broad detection limits and high linearity. Low background and elevated measurement ceilings allow precise quantification of low-abundance targets, outperforming conventional detection methods.
3.Enhanced Stability and Reproducibility:Formulated with proprietary chelators and detergents, the solution maintains stable performance under recommended storage (4 °C). It minimizes interference from contaminants and ensures consistent results across different batches and experiments.
4.Simple and Streamlined Workflow:Ready-to-use and compatible with standard TRF-capable microplate readers, the solution integrates seamlessly into existing protocols. After addition, only brief shaking and incubation are required—no wash steps—making it ideal for high-throughput screening and routine use.
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