Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
Email Us

TR FRET Assay Kit

VKEY-BIO's TR-FRET assay kits utilize advanced Time-Resolved Fluorescence Resonance Energy Transfer technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.

Get a Free Quote
Tool-Box
Tool-Box
Protein-protein interaction is fundamental to signal transduction both within and between cells, as well as to metabolic regulation and biological reactions. The KeyTec® TR-FRET Toolbox series offers a straightforward and rapid means of detecting protein-protein interactions.
Biomarker
Biomarker
Biomarker detection includes cAMP detection and cytokine (TNFα, IL6) detection. cAMP detection is helpful for further exploring the mechanism of cell signal transduction and better understanding the regulatory process of cell physiological functions.
Biotherapeutics
Biotherapeutics
Biopharmaceutical - related detection products include antibody quantification detection kits and hybridoma screening kits. The KeyTec® TR - FRET Human antibody quantification series kits, by using the TR - FRET technology, simplify the entire detection process into "sample loading - incubation - plate reading".
Immunity & Cancer
Immunity & Cancer
Using the KeyTec® TR-FRET technology for high-throughput screening of small-molecule inhibitors, polypeptides, or antibody blockers offers several distinct advantages. This technology is characterized by its simplicity, allowing researchers to easily set up.
Kinase Assay
Kinase Assay
The KeyTec® TR-FRET Kinase Series provides a highly efficient and precise solution for kinase activity detection. This product line includes assay kits and specific kinase substrates for kinases, tyrosine kinases (TKS), and cyclin-dependent kinases (CDKs). Utilizing advanced TR-FRET technology, the KeyTec® TR-FRET Kinase Series combines specific substrates with phosphorylation antibodies to achieve highly sensitive and specific detection of kinase activity based on the principle of fluorescence resonance energy transfer. With its user-friendly operation, excellent reproducibility, and flexible applications, the KeyTec® TR-FRET Kinase Series is widely used in drug screening and fundamental research.
TR-FRET Assay Working Principle

TR-FRET Assay Working Principle

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). By utilizing the long fluorescence lifetime and high stability of lanthanide elements, our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity.

Advantages of VKEY-BIO's TR-FRET Assay Kits

Easy to Use
Easy to Use

TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.

Good Specificity and High Sensitivity
Good Specificity and High Sensitivity

The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.

Suitable for High Throughput Screening
Suitable for High Throughput Screening

Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.

Good Stability and Repeatability
Good Stability and Repeatability

VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.