KeyTec® TR-FRET Detection Assay Kit

Protein-protein interaction is fundamental to signal transduction both within and between cells, as well as to metabolic regulation and biological reactions. The KeyTec® TR-FRET Toolbox series offers a straightforward and rapid means of detecting protein-protein interactions.

Biomarker

Biomarker detection includes cAMP detection and cytokine (TNFα, IL6) detection. cAMP detection is helpful for further exploring the mechanism of cell signal transduction and better understanding the regulatory process of cell physiological functions.

Biotherapeutics

Biopharmaceutical - related detection products include antibody quantification detection kits and hybridoma screening kits. The KeyTec® TR - FRET Human antibody quantification series kits, by using the TR - FRET technology, simplify the entire detection process into "sample loading - incubation - plate reading".

Immunity & Cancer

Using the KeyTec® TR-FRET technology for high-throughput screening of small-molecule inhibitors, polypeptides, or antibody blockers offers several distinct advantages. This technology is characterized by its simplicity, allowing researchers to easily set up.

Kinase Assay

The KeyTec® TR-FRET Kinase Series provides a highly efficient and precise solution for kinase activity detection. This product line includes assay kits and specific kinase substrates for kinases, tyrosine kinases (TKS), and cyclin-dependent kinases (CDKs). Utilizing advanced TR-FRET technology, the KeyTec® TR-FRET Kinase Series combines specific substrates with phosphorylation antibodies to achieve highly sensitive and specific detection of kinase activity based on the principle of fluorescence resonance energy transfer. With its user-friendly operation, excellent reproducibility, and flexible applications, the KeyTec® TR-FRET Kinase Series is widely used in drug screening and fundamental research.

GPCR

A1030003S A1030003L A1030003B

General Reagent

A1010001S A1010001L A1010001B

TR-FRET Assay Working Principle

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.