Time-Resolved Fluorescence (TRF) Analysis: This method, often referred to as TRF fluorescence analysis, is based on the long fluorescence effect of lanthanide chelates and involves fluorescence measurements from both time and intensity dimensions. Since its establishment in the 1980s, time resolved fluorescence TRF technology has effectively eliminated the interference of non-specific fluorescence, significantly enhancing analytical sensitivity.
Eu3+/Sm3+ labeling reagents are used to label macromolecules like proteins, peptides, and oligonucleotides, and the resulting markers are suitable for various types of analysis based on solid phase separations. After a series of manipulation steps, the remaining Eu3+/Sm3+-containing marker is immobilized onto a microtiter plate after the unbound portion is washed away. This solution is then added and can dissociate Eu3+/Sm3+ from the immobilized labeled antibody or protein within a few minutes, forming a homogeneous time-resolved fluorescent micellar chelate solution with extremely high brightness. highly sensitive measurements of analytes can be performed.. It is compatible with DELFIA assay.
Immunoassays (TRFIA/DELFIA):
TRF technology is widely used in ultrasensitive immunoassays, such as TR-FLISA and DELFIA, to detect biomarkers, hormones, and infectious agents. By utilizing lanthanide chelates (e.g., Eu³⁺/Sm³⁺), it eliminates short-lived background fluorescence, enabling high signal-to-noise ratios for clinical and research diagnostics.
Drug Discovery & High-Throughput Screening (HTS):
TRF-based assays (e.g., TR-FRET) are critical in HTS for studying protein-protein interactions, enzyme activity, and receptor binding. The long fluorescence lifetime allows gated detection, reducing interference from compound autofluorescence.
Nucleic Acid Detection:
TRF probes quantify DNA/RNA with exceptional sensitivity, applied in PCR, hybridization assays, and SNP genotyping. Lanthanide-labeled oligonucleotides enhance stability and detection limits.
Cell Imaging & Microscopy:
Time-resolved fluorescence imaging visualizes subcellular structures and dynamic processes (e.g., receptor trafficking) by suppressing autofluorescence, ideal for live-cell studies.
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