KeyTec® TR-FRET BRD4 / AR RIPTAC Binding Assay kit
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CAT.: A1090019D
The KeyTec® TR-FRET BRD4/AR RIPTAC Binding Assay Kit can be used to screen RIPTAC molecules that mediate the binding of BRD4 protein and AR protein to form a ternary complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and good reproducibility.
The basic principle of this method is shown in the figure below. The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody specifically recognizes Tag1-BRD4 protein, and the KeyTec® TR-FRET LA2-conjugated anti-Tag2 antibody specifically recognizes Tag2-AR protein. The Tag1-BRD4 protein complex and Tag2-AR protein can form a ternary complex mediated by the positive control RIPTAC molecule AR/BRD4 RIPTAC-1, thereby bringing Solar Eu and LA into proximity. Upon excitation by an external light source, resonance energy transfer occurs between the donor and acceptor. Measuring the signal intensity at a specific wavelength (665 nm) allows determination of RIPTAC-mediated binding between AR and BRD4.
Standard curve
Comparison of different incubation times
| CAT. | Description | Size |
| A1090019D | KeyTec® TR-FRET BRD4 / AR RIPTAC Binding Assay kit | 100 tests |
|
• 1 vial (8 μL/vial) Tag2-AR • 1 vial (8 μL/vial) Tag1-BRD4 • 1 vial (5 μL/vial) mAb anti-Tag1 - Solar Eu • 1 vial (5 μL/vial) mAb anti-Tag2 - LA • 2 vials (2 mL/vial) PROTAC Diluent Buffer #2 • 1 vial (2 mL/vial) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET BRD4/AR RIPTAC Binding Assay Kit can be used to screen RIPTAC molecules that mediate the binding of BRD4 protein and AR protein to form a ternary complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and good reproducibility.
The basic principle of this method is shown in the figure below. The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody specifically recognizes Tag1-BRD4 protein, and the KeyTec® TR-FRET LA2-conjugated anti-Tag2 antibody specifically recognizes Tag2-AR protein. The Tag1-BRD4 protein complex and Tag2-AR protein can form a ternary complex mediated by the positive control RIPTAC molecule AR/BRD4 RIPTAC-1, thereby bringing Solar Eu and LA into proximity. Upon excitation by an external light source, resonance energy transfer occurs between the donor and acceptor. Measuring the signal intensity at a specific wavelength (665 nm) allows determination of RIPTAC-mediated binding between AR and BRD4.
Standard curve
Comparison of different incubation times
| CAT. | Description | Size |
| A1090019S | KeyTec® TR-FRET BRD4 / AR RIPTAC Binding Assay kit | 500 tests |
|
• 1 vial (20 μL/vial) RIPTAC Standard • 1 vial (40 μL/vial) Tag2-AR • 1 vial (40 μL/vial) Tag1-BRD4 • 1 vial (25 μL/vial) mAb anti-Tag1 - Solar Eu • 1 vial (25 μL/vial) mAb anti-Tag2 - LA • 1 bottle (50 mL/bottle) PROTAC Diluent Buffer #2 • 1 bottle (30 mL/bottle) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET BRD4/AR RIPTAC Binding Assay Kit can be used to screen RIPTAC molecules that mediate the binding of BRD4 protein and AR protein to form a ternary complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and good reproducibility.
The basic principle of this method is shown in the figure below. The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody specifically recognizes Tag1-BRD4 protein, and the KeyTec® TR-FRET LA2-conjugated anti-Tag2 antibody specifically recognizes Tag2-AR protein. The Tag1-BRD4 protein complex and Tag2-AR protein can form a ternary complex mediated by the positive control RIPTAC molecule AR/BRD4 RIPTAC-1, thereby bringing Solar Eu and LA into proximity. Upon excitation by an external light source, resonance energy transfer occurs between the donor and acceptor. Measuring the signal intensity at a specific wavelength (665 nm) allows determination of RIPTAC-mediated binding between AR and BRD4.
Standard curve
Comparison of Different Incubation Times
| CAT. | Description | Size |
| A1090019L | KeyTec® TR-FRET BRD4 / AR RIPTAC Binding Assay kit | 5,000 tests |
|
• 2 vials (20 μL/vial) RIPTAC Standard • 1 vial (400 μL/vial) Tag2-AR • 1 vial (400 μL/vial) Tag1-BRD4 • 1 vial (250 μL/vial) mAb anti-Tag1 - Solar Eu • 1 vial (250 μL/vial) mAb anti-Tag2 - LA • 1 bottle (200 mL/bottle) PROTAC Diluent Buffer #2 • 1 bottle (120 mL/bottle) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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