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KeyTec® TR-FRET Human PARG inhibitor screening kit

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KeyTec® TR-FRET Human PARG inhibitor screening kit

Size:

Price: $ Inquire

CAT.: A1090006D

KeyTec® TR-FRET Human PARG inhibitor screening kit
A1090006S
A1090006S
A1090006S
微信图片_20240227101945
微信图片_20240227101945
微信图片_20240227101945
Size:
100 tests
500 tests
5,000 tests
Price:
$ Inquire
CAT.:
A1090006D
Delivery:
Available in Stock
Price:
$ Inquire
CAT.:
A1090006S
Delivery:
Available in Stock
Price:
$ Inquire
CAT.:
A1090006L
Delivery:
Available in Stock
Sample Request
KeyTec® TR-FRET Human PARG inhibitor screening kit
100 tests
100 tests
500 tests
5,000 tests
CAT.: A1090006D
$ Inquire $ Inquire $ Inquire
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® TR-FRET Human PARG Inhibitor Screening Kit is designed for screening inhibitors of the PARG enzyme. This kit employs an immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.

The basic principle of this method is illustrated in the figure below. The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody and the KeyTec® TR-FRET LA2-conjugated Streptavidin (SA) can bind to the Tag1-tagged Biotin-PARylated protein substrate, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor, allowing detection of signal intensity at a specific wavelength (665 nm). The PARG enzyme hydrolyzes the biotinylated poly(ADP-ribose) chains attached to the protein substrate. When the system contains a PARG enzyme inhibitor, the hydrolysis activity of PARG is impeded, and the TR-FRET signal intensity is directly proportional to the inhibitor concentration in the sample.


KeyTec® TR-FRET Human PARG inhibitor screening kit A1090006D

Performance

A1090006_1.2

                                                                    control sample data

Components

CAT. Description Size
A1090006D KeyTec® TR-FRET Human PARG inhibitor screening kit 100 tests

  •  1 vial （5 μL/vial） mAb anti-Tag1- Solar Eu (400X）
  •  1 vial （5 μL/vial） Streptavidin– LA (400X）
  •  1 vial （5 μL/vial） Biotin-PARlated substrate(32X）
  •  1 vial （5 μL/vial） PARG (8000X）
  •  1 vial （5 μL/vial） PARG Inhibitor， [PDD00017273] (10 mM)
  •  1 vial （5 μL of 1M solution） DTT Lyophilized
  •  1 vial （2 mL/vial） PARG Reaction Buffer (1X）
  •  1 bottle （ 2 mL/bottle A1010002D）Solar Eu Detection Buffer (1X)

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

Overview

KeyTec® TR-FRET Human PARG Inhibitor Screening Kit is designed for screening inhibitors of the PARG enzyme. This kit employs an immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.

The basic principle of this method is illustrated in the figure below. The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody and the KeyTec® TR-FRET LA2-conjugated Streptavidin (SA) can bind to the Tag1-tagged Biotin-PARylated protein substrate, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor, allowing detection of signal intensity at a specific wavelength (665 nm). The PARG enzyme hydrolyzes the biotinylated poly(ADP-ribose) chains attached to the protein substrate. When the system contains a PARG enzyme inhibitor, the hydrolysis activity of PARG is impeded, and the TR-FRET signal intensity is directly proportional to the inhibitor concentration in the sample.


A1090006_1.1

Performance

A1090006_1.2

                                                                          control sample data

Components

CAT. Description Size
A1090006S KeyTec® TR-FRET Human PARG inhibitor screening kit 500 tests

  •  1 vial （25 μL/vial） mAb anti-Tag1- Solar Eu (400X）
  •  1 vial （25 μL/vial） Streptavidin– LA (400X）
  •  1 vial （25 μL/vial） Biotin-PARlated substrate(32X）
  •  1 vial （25 μL/vial） PARG (8000X）
  •  1 vial （25 μL/vial） PARG Inhibitor， [PDD00017273] (10 mM)
  •  1 vial （120 µ moles/vial A1010022S） DTT Lyophilized
  •  1 bottle （ 50 mL/bottle） PARG Reaction Buffer (1X）
  •  1 bottle （ 30 mL/bottle A1010002S）Solar Eu Detection Buffer (1X)

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

Overview

KeyTec® TR-FRET Human PARG Inhibitor Screening Kit is designed for screening inhibitors of the PARG enzyme. This kit employs an immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.

The basic principle of this method is illustrated in the figure below. The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody and the KeyTec® TR-FRET LA2-conjugated Streptavidin (SA) can bind to the Tag1-tagged Biotin-PARylated protein substrate, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor, allowing detection of signal intensity at a specific wavelength (665 nm). The PARG enzyme hydrolyzes the biotinylated poly(ADP-ribose) chains attached to the protein substrate. When the system contains a PARG enzyme inhibitor, the hydrolysis activity of PARG is impeded, and the TR-FRET signal intensity is directly proportional to the inhibitor concentration in the sample.



A1090006_1.1

Performance

A1090006_1.2

                                                                       control sample data

Components

CAT. Description Size
A1090006L KeyTec® TR-FRET Human PARG inhibitor screening kit 5,000 tests

  •  1 vial （250 μL/vial） mAb anti-Tag1- Solar Eu (400X）
  •  1 vial （250 μL/vial） Streptavidin– LA (400X）
  •  1 vial （250 μL/vial） Biotin-PARlated substrate(32X）
  •  2 vials （25 μL/vial） PARG (8000X）
  •  2 vials （25 μL/vial） PARG Inhibitor， [PDD00017273] (10 mM)
  •  1 vial （120 µ moles/vial A1010022S） DTT Lyophilized
  •  1 bottle （ 200 mL/bottle） PARG Reaction Buffer (1X）
  •  1 bottle （ 120 mL/bottle A1010002L）Solar Eu Detection Buffer (1X)

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

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