KeyTec® TR-FRET PD1/PD-L2 Binding Assay kit
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CAT.: A1090014D
The KeyTec® TR‑FRET Human PD1/PD‑L2 Binding Assay Kit is designed to measure the interaction between PD1 and PD‑L2 proteins. This kit is based on KeyTec® TR‑FRET technology and is suitable for high‑throughput screening of small‑molecule inhibitors, peptides, or antibody blockers, featuring simple operation, rapid performance, and high accuracy.
As illustrated in the schematic diagram below, a Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes Tag1‑tagged PD‑L2 protein, while an LA2‑conjugated anti‑Tag2 antibody recognizes Tag2‑tagged PD1 protein. When PD‑L2 binds to PD1, the respective tag antibodies conjugated with Solar Eu and LA are brought into close proximity, allowing energy resonance transfer between the donor and acceptor upon external light excitation. The degree of interaction between PD1 and PD‑L2 is then determined by measuring the signal intensity at 665 nm. Consequently, compounds, peptides, or antibodies that block the PD1/PD‑L2 interaction will cause a decrease in the TR‑FRET signal.
Validation results with standard blocker Anti‑PD1
Test results of the standard in different matrix solutions
Test results at different incubation times
| CAT. | Description | Size |
| A1090014D | KeyTec® TR-FRET PD1/PD-L2 Binding Assay kit | 100 tests |
|
• 1 vial(8 μL/vial) Tag1-PD-L2 • 1 vial(8 μL/vial) Tag2-PD1 • 1 vial(5 μL/vial) Anti-Tag1–Solar Eu • 1 vial(5 μL/vial) Anti-Tag2–LA • 2 vials(2 mL/vial) Binding Assay Diluent Buffer • 1 vial(2 mL/vial) Binding Assay Diluent Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR‑FRET Human PD1/PD‑L2 Binding Assay Kit is designed to measure the interaction between PD1 and PD‑L2 proteins. This kit is based on KeyTec® TR‑FRET technology and is suitable for high‑throughput screening of small‑molecule inhibitors, peptides, or antibody blockers, featuring simple operation, rapid performance, and high accuracy.
As illustrated in the schematic diagram below, a Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes Tag1‑tagged PD‑L2 protein, while an LA2‑conjugated anti‑Tag2 antibody recognizes Tag2‑tagged PD1 protein. When PD‑L2 binds to PD1, the respective tag antibodies conjugated with Solar Eu and LA are brought into close proximity, allowing energy resonance transfer between the donor and acceptor upon external light excitation. The degree of interaction between PD1 and PD‑L2 is then determined by measuring the signal intensity at 665 nm. Consequently, compounds, peptides, or antibodies that block the PD1/PD‑L2 interaction will cause a decrease in the TR‑FRET signal.
Validation results with standard blocker Anti‑PD1
Test results of the standard in different matrix solutions
Test results at different incubation times
| CAT. | Description | Size |
| A1090014S | KeyTec® TR-FRET PD1/PD-L2 Binding Assay kit | 500 tests |
|
• 1 vial(40 μL/vial) Tag1-PD-L2 • 1 vial(40 μL/vial) Tag2-PD1 • 1 vial(25 μL/vial) Anti-Tag1–Solar Eu • 1 vial(25 μL/vial) Anti-Tag2–LA • 1 bottle(50 mL/bottle) Binding Assay Diluent Buffer • 1 bottle(30 mL/bottle) Binding Assay Diluent Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR‑FRET Human PD1/PD‑L2 Binding Assay Kit is designed to measure the interaction between PD1 and PD‑L2 proteins. This kit is based on KeyTec® TR‑FRET technology and is suitable for high‑throughput screening of small‑molecule inhibitors, peptides, or antibody blockers, featuring simple operation, rapid performance, and high accuracy.
As illustrated in the schematic diagram below, a Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes Tag1‑tagged PD‑L2 protein, while an LA2‑conjugated anti‑Tag2 antibody recognizes Tag2‑tagged PD1 protein. When PD‑L2 binds to PD1, the respective tag antibodies conjugated with Solar Eu and LA are brought into close proximity, allowing energy resonance transfer between the donor and acceptor upon external light excitation. The degree of interaction between PD1 and PD‑L2 is then determined by measuring the signal intensity at 665 nm. Consequently, compounds, peptides, or antibodies that block the PD1/PD‑L2 interaction will cause a decrease in the TR‑FRET signal.
Validation results with standard blocker Anti‑PD1
Test results of the standard in different matrix solutions
Test results at different incubation times
| CAT. | Description | Size |
| A1090014L | KeyTec® TR-FRET PD1/PD-L2 Binding Assay kit | 5,000 tests |
|
• 1 vial(400 μL/vial) Tag1-PD-L2 • 1 vial(400 μL/vial) Tag2-PD1 • 1 vial(250 μL/vial) Anti-Tag1–Solar Eu • 1 vial(250 μL/vial) Anti-Tag2–LA • 1 bottle(200 mL/bottle) Binding Assay Diluent Buffer • 1 bottle(120 mL/bottle) Binding Assay Diluent Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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