KeyTec® Biotin-LATS1／MOBKL1A, N-Flag-tag-; C-Avi/N-His
Size: Used for Developing Biotin-LATS1／MOBKL1A Biochemical Activity Assay or Binding Assay Models
Price: $ Inquire
CAT.: P1HI0164S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
AA Sequences Uniprot: LATS1: O95835-1, K589-V1130(end); MOBKL1A: Q7L9L4-1, M1-R216(end)
Tag LATS1: N-terminal Flag tag, C-terminal Avi tag; MOBKL1A: N-terminal 6x His tag
Molecular Weight LATS1 66.5 kDa; MOBKL1A: 26.2 kDa
Species Human
Expression Host Sf9
Purity 0.36 mg/mL by OD280
Protein Concentration >95% by SDS-PAGE
Form Liquid
Formulation 50M Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
This Biotin-LATS1／MOBKL1A recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating Biotin-LATS1／MOBKL1A protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0164S | KeyTec® Biotin-LATS1／MOBKL1A, N-Flag-tag-; C-Avi/N-His | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
AA Sequences Uniprot: LATS1: O95835-1, K589-V1130(end); MOBKL1A: Q7L9L4-1, M1-R216(end)
Tag LATS1: N-terminal Flag tag, C-terminal Avi tag; MOBKL1A: N-terminal 6x His tag
Molecular Weight LATS1 66.5 kDa; MOBKL1A: 26.2 kDa
Species Human
Expression Host Sf9
Purity 0.36 mg/mL by OD280
Protein Concentration >95% by SDS-PAGE
Form Liquid
Formulation 50M Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
This Biotin-LATS1／MOBKL1A recombinant protein was verified using the ADP-Glo assay and showed high enzymatic activity. The enzymatic reaction was performed by incubating Biotin-LATS1／MOBKL1A protein, ATP, and substrate at 25 °C for 60 minutes, followed by the ADP-Glo Kinase Assay measures ADP formed from the enzymatic reaction. The luminescent signal was measured using the Chemiluminescent module of a microplate reader.
| CAT. | Description | Size |
| P1HI0164L | KeyTec® Biotin-LATS1／MOBKL1A, N-Flag-tag-; C-Avi/N-His | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
English
