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KeyTec® EGFR[L858R/T790M], N-GST

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KeyTec® EGFR[L858R/T790M], N-GST

Size: Used for Developing EGFR[L858R/T790M] Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0038S

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Used for Developing EGFR[L858R/T790M] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR[L858R/T790M] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR[L858R/T790M] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR[L858R/T790M] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0038S
Delivery:
2-3 weeks
Price:
$ 3990
CAT.:
P1HI0038L
Delivery:
2-3 weeks
Price:
$ 661
CAT.:
P1HI0212S
Delivery:
2-3 weeks
Price:
$ 3990
CAT.:
P1HI0212L
Delivery:
2-3 weeks
KeyTec® EGFR[L858R/T790M], N-GST
10 μg
10 μg
100 μg
10 μg
100 μg
CAT.: P1HI0038S
$ 660 $ 3990 $ 661 $ 3990
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® EGFR[L858R/T790M], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The EGFR[L858R/T790M] protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P00533-1; R669-A1210(end), L858R, T790M
Tag： N-terminal GST tag
Molecular Weight： 87.8 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.32 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% Glycerol, pH7.5

Performance

P1HI0038_2.1.png
P1HI0038_2.2.png
The EGFR[L858R/T790M] activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR[L858R/T790M] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0038_2.3.png

Protocols

/uploads/file/product/COA_P1HI0038_K130323012_EN.pdf
/uploads/file/product/COA_P1HI0038_K130323012_EN.pdf Download

Components

CAT. Description Size
P1HI0038S KeyTec® EGFR[L858R/T790M], N-GST 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® EGFR[L858R/T790M], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The EGFR[L858R/T790M] protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P00533-1; R669-A1210(end), L858R, T790M
Tag： N-terminal GST tag
Molecular Weight： 87.8 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.32 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% Glycerol, pH7.5

Performance

P1HI0038_2.1.png
P1HI0038_2.2.png
The EGFR[L858R/T790M] activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR[L858R/T790M] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0038_2.3.png

Protocols

/uploads/file/product/COA_P1HI0038_K130323012_EN.pdf
/uploads/file/product/COA_P1HI0038_K130323012_EN.pdf Download

Components

CAT. Description Size
P1HI0038L KeyTec® EGFR[L858R/T790M], N-GST 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

Please contact sales for details

Performance

Components

CAT. Description Size
P1HI0212S KeyTec® EGFR[L858R/T790M], N-GST 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

Please contact sales for details

Performance

Components

CAT. Description Size
P1HI0212L KeyTec® EGFR[L858R/T790M], N-GST 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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