KeyTec® TR-FRET Human IL12/IL12R-β1 Binding Assay Kit
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CAT.: A1070105D
The KeyTec® Human TR-FRET IL12/IL12R-β1 Binding Assay Kit is designed for high-throughput screening of small-molecule inhibitors, peptides, or antibodies that block the interaction between IL12 and IL12R-β1 proteins. This kit utilizes KeyTec® TR-FRET technology, offering advantages such as simplicity, speed, and high accuracy.
As illustrated in the principle diagram below, the KeyTec® TR-FRET Solar Eu®¹-labeled anti-Tag 1 antibody specifically recognizes IL12 protein carrying Tag 1, while the KeyTec® TR-FRET LA®²-labeled Streptavidin recognizes biotinylated IL12R-β1 protein (AVI-Biotin). When IL12 binds to IL12R-β1, Solar Eu and LA are brought into close proximity, enabling energy resonance transfer between the donor and acceptor upon external light excitation. The interaction level between IL12 and IL12R-β1 is determined by measuring the signal intensity at a specific wavelength (665 nm). Consequently, compounds, peptides, or antibodies that disrupt the IL12–IL12R-β1 interaction will result in a decrease in TR-FRET signal.
Standard Blocker Validation Results
Test Results of Different Blockers
| CAT. | Description | Size |
| A1070105D | KeyTec® TR-FRET Human IL12/IL12R-β1 Binding Assay Kit | 100 tests |
|
• 1 vial of 20 µL/vial IL12 / IL12R-β1 binding standard • 1 vial of 8 µL/vial Tag1-IL12 • 1 vial of 8 µL/vial IL12R-β1-Biotin • 1 vial of 5 µL/vial Anti-Tag1–Solar Eu • 1 vial of 5 µL/vial Streptavidin–LA • 2 vials 2 mL/vial Binding Assay Diluent Buffer • 1 vial 2 mL/vial Solar Eu Detection Buffer |
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| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
The KeyTec® Human TR-FRET IL12/IL12R-β1 Binding Assay Kit is designed for high-throughput screening of small-molecule inhibitors, peptides, or antibodies that block the interaction between IL12 and IL12R-β1 proteins. This kit utilizes KeyTec® TR-FRET technology, offering advantages such as simplicity, speed, and high accuracy.
As illustrated in the principle diagram below, the KeyTec® TR-FRET Solar Eu®¹-labeled anti-Tag 1 antibody specifically recognizes IL12 protein carrying Tag 1, while the KeyTec® TR-FRET LA®²-labeled Streptavidin recognizes biotinylated IL12R-β1 protein (AVI-Biotin). When IL12 binds to IL12R-β1, Solar Eu and LA are brought into close proximity, enabling energy resonance transfer between the donor and acceptor upon external light excitation. The interaction level between IL12 and IL12R-β1 is determined by measuring the signal intensity at a specific wavelength (665 nm). Consequently, compounds, peptides, or antibodies that disrupt the IL12–IL12R-β1 interaction will result in a decrease in TR-FRET signal.
Standard Blocker Validation Results
Test Results of Different Blockers
| CAT. | Description | Size |
| A1070105S | KeyTec® TR-FRET Human IL12/IL12R-β1 Binding Assay Kit | 500 tests |
|
• 1 vial of 50 µL/vial IL12 / IL12R-β1 binding standard • 1 vial of 40 µL/vial Tag1-IL12 • 1 vial of 40 µL/vial IL12R-β1-Biotin • 1 vial of 25 µL/vial Anti-Tag1–Solar Eu • 1 vial of 25 µL/vial Streptavidin–LA • 1 bottle 50 mL/bottle Binding Assay Diluent Buffer • 1 bottle 30 mL/bottle Solar Eu Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
The KeyTec® Human TR-FRET IL12/IL12R-β1 Binding Assay Kit is designed for high-throughput screening of small-molecule inhibitors, peptides, or antibodies that block the interaction between IL12 and IL12R-β1 proteins. This kit utilizes KeyTec® TR-FRET technology, offering advantages such as simplicity, speed, and high accuracy.
As illustrated in the principle diagram below, the KeyTec® TR-FRET Solar Eu®¹-labeled anti-Tag 1 antibody specifically recognizes IL12 protein carrying Tag 1, while the KeyTec® TR-FRET LA®²-labeled Streptavidin recognizes biotinylated IL12R-β1 protein (AVI-Biotin). When IL12 binds to IL12R-β1, Solar Eu and LA are brought into close proximity, enabling energy resonance transfer between the donor and acceptor upon external light excitation. The interaction level between IL12 and IL12R-β1 is determined by measuring the signal intensity at a specific wavelength (665 nm). Consequently, compounds, peptides, or antibodies that disrupt the IL12–IL12R-β1 interaction will result in a decrease in TR-FRET signal.
Standard Blocker Validation Results
Test Results of Different Blockers
| CAT. | Description | Size |
| A1070105L | KeyTec® TR-FRET Human IL12/IL12R-β1 Binding Assay Kit | 5,000 tests |
|
• 2 vials of 50 µL/vial IL12 / IL12R-β1 binding standard • 1 vial of 400 µL/vial Tag1-IL12 • 1 vial of 400 µL/vial IL12R-β1-Biotin • 1 vial of 250 µL/vial Anti-Tag1–Solar Eu • 1 vial of 250 µL/vial Streptavidin–LA • 1 bottle 200 mL/bottle Binding Assay Diluent Buffer • 1 bottle 120 mL/bottle Solar Eu Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
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