KeyTec® TR-FRET Human IL17AA / IL17RA Binding Assay Kit
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CAT.: A1070102D
The KeyTec® TR-FRET Human IL17AA/IL17RA Binding Assay Kit is designed for measuring the interaction between IL17A and IL17RA proteins. Employing KeyTec® TR-FRET technology, this kit is suitable for high‑throughput screening of small‑molecule inhibitors, peptides, or antibody blockers, and is characterized by simplicity, rapidity, and high accuracy.
The basic principle of this method is as follows (as shown in the figure below): KeyTec® TR-FRET Solar Eu‑conjugated Streptavidin and biotinylated Human IL17A protein (AVI‑Biotin) are pre‑bound to form a complex, while KeyTec® TR‑FRET LA‑conjugated IL17RA protein is also provided. When IL17A binds to IL17RA, the respective tag antibodies or affinity reagents conjugated with Solar Eu and LA are brought into close proximity. Upon excitation by an external light source, resonance energy transfer occurs between the donor and the acceptor. Detection of the signal intensity at a specific wavelength (665 nm) allows quantification of the degree of interaction between IL17A and IL17RA proteins. Consequently, compounds, peptides, or antibodies that block the IL17A‑IL17RA interaction will cause a decrease in the TR‑FRET signal.
Standard curve
| CAT. | Description | Size |
| A1070102D | KeyTec® TR-FRET Human IL17AA / IL17RA Binding Assay Kit | 100 tests |
|
• 1 vial of 20 µL IL17 / IL17RA binding Standard • 1 vial of 5 µL Biotin- IL17AA& SA- Solar Eu Mix • 1 vial of 5 µL IL17RA-LA • 2 vials of 2 mL Binding Assay Diluent Buffer • 1 vial of 2 mL Solar Eu Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
The KeyTec® TR-FRET Human IL17AA/IL17RA Binding Assay Kit is designed for measuring the interaction between IL17A and IL17RA proteins. Employing KeyTec® TR-FRET technology, this kit is suitable for high‑throughput screening of small‑molecule inhibitors, peptides, or antibody blockers, and is characterized by simplicity, rapidity, and high accuracy.
The basic principle of this method is as follows (as shown in the figure below): KeyTec® TR-FRET Solar Eu‑conjugated Streptavidin and biotinylated Human IL17A protein (AVI‑Biotin) are pre‑bound to form a complex, while KeyTec® TR‑FRET LA‑conjugated IL17RA protein is also provided. When IL17A binds to IL17RA, the respective tag antibodies or affinity reagents conjugated with Solar Eu and LA are brought into close proximity. Upon excitation by an external light source, resonance energy transfer occurs between the donor and the acceptor. Detection of the signal intensity at a specific wavelength (665 nm) allows quantification of the degree of interaction between IL17A and IL17RA proteins. Consequently, compounds, peptides, or antibodies that block the IL17A‑IL17RA interaction will cause a decrease in the TR‑FRET signal.
Standard curve
| CAT. | Description | Size |
| A1070102S | KeyTec® TR-FRET Human IL17AA / IL17RA Binding Assay Kit | 500 tests |
|
• 1 vial of 50 µL IL17 / IL17RA binding Standard • 1 vial of 25 µL Biotin- IL17AA& SA- Solar Eu Mix • 1 vial of 25 µL IL17RA-LA • 1 bottle of 50 mL (A1010001S) Binding Assay Diluent Buffer • 1 bottle of 30 mL (A1010002S) Solar Eu Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
The KeyTec® TR-FRET Human IL17AA/IL17RA Binding Assay Kit is designed for measuring the interaction between IL17A and IL17RA proteins. Employing KeyTec® TR-FRET technology, this kit is suitable for high‑throughput screening of small‑molecule inhibitors, peptides, or antibody blockers, and is characterized by simplicity, rapidity, and high accuracy.
The basic principle of this method is as follows (as shown in the figure below): KeyTec® TR-FRET Solar Eu‑conjugated Streptavidin and biotinylated Human IL17A protein (AVI‑Biotin) are pre‑bound to form a complex, while KeyTec® TR‑FRET LA‑conjugated IL17RA protein is also provided. When IL17A binds to IL17RA, the respective tag antibodies or affinity reagents conjugated with Solar Eu and LA are brought into close proximity. Upon excitation by an external light source, resonance energy transfer occurs between the donor and the acceptor. Detection of the signal intensity at a specific wavelength (665 nm) allows quantification of the degree of interaction between IL17A and IL17RA proteins. Consequently, compounds, peptides, or antibodies that block the IL17A‑IL17RA interaction will cause a decrease in the TR‑FRET signal.
Standard curve
| CAT. | Description | Size |
| A1070102L | KeyTec® TR-FRET Human IL17AA / IL17RA Binding Assay Kit | 5,000 tests |
|
• 2 vials of 50 µL IL17 / IL17RA binding Standard • 1 vial of 250 µL Biotin- IL17AA & SA- Solar Eu Mix • 1 vial of 250 µL IL17RA-LA • 1 bottle of 200 mL (A1010001L) Binding Assay Diluent Buffer • 1 bottle of 120 mL (A1010002L) Solar Eu Detection Buffer |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60 ℃及以下 |
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