Protein-protein interaction is fundamental to signal transduction both within and between cells, as well as to metabolic regulation and biological reactions. Consequently, in-depth studies of these interactions not only enhance our understanding of intracellular biological mechanisms but also hold significant importance for drug research and development, as well as disease treatment. The KeyTec® TR-FRET Toolbox series offers a straightforward and rapid means of detecting protein-protein interactions, making it an excellent choice for drug screening and research into drug action mechanisms.
KeyTec® TR-FRET Streptavidin-Solar Eu is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the donor (KeyTec® TR-FRET Streptavidin-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-Solar Tb is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the donor (KeyTec® TR-FRET Streptavidin-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-LA is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the acceptor (KeyTec® TR-FRET Streptavidin- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-HX is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the acceptor (KeyTec® TR-FRET Streptavidin-HX*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET pAb anti-Human IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Human IgG antibody is a mouse polyclonal antibody. In the Antigen-Antibody Interaction assay, the Human IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Human IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Human IgG-LA is designed for developing the TR-FRET Assay. The anti- Human IgG antibody is a mouse polyclonal antibody. In the Antigen-Antibody Interaction assay, the Human IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Human IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Human IgG-HX is designed for developing the TR-FRET Assay. The anti- Human IgG antibody is a mouse polyclonal antibody. In the Antigen-Antibody Interaction assay, the Human IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Human IgG-HX*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Mouse IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Mouse IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Mouse IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Mouse IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Mouse IgG-LA is designed for developing the TR-FRET Assay. The anti-Mouse IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Mouse IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Mouse IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Rabbit IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Rabbit IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rabbit IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Rabbit IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Rabbit IgG-LA is designed for developing the TR-FRET Assay. The anti-Rabbit IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rabbit IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Rabbit IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Rat IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Rat IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rat IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Rat IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Rat IgG-LA is designed for developing the TR-FRET Assay. The anti-Rat IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rat IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Rat IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET Protein A-Solar Eu is designed for developing the TR-FRET Assay. Protein A has high affinity for Ig types from different species, particularly IgG. In the Antigen-Antibody Interaction assay, the IgG antibody binds to the donor (KeyTec® TR-FRET Protein A-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET Protein A-LA is designed for developing the TR-FRET Assay. Protein A exhibits high affinity towards various species of Ig, particularly IgG. In the Antigen-Antibody Interaction assay, the IgG antibody binds to the acceptor (KeyTec® TR-FRET Protein A-LA*2), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu*1). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
The KeyTec® TR - FRET Tool - Box series of products provide flexible solutions for developing protein - protein interaction experiments. This series includes tag antibodies (such as Anti - HIS, Anti - GST, etc.), secondary antibodies (such as Anti - Human IgG, etc.) and proteins (Streptavidin, Protein A, etc.) conjugated with TR-FRET fluorescence resonance energy transfer donors (Solar Eu/Solar Tb) or fluorescence resonance energy transfer acceptors (LA/HX), allowing you to develop your own TR-FRET experiments like 'building blocks'.
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