Protein-protein interaction is fundamental to signal transduction both within and between cells, as well as to metabolic regulation and biological reactions. Consequently, in-depth studies of these interactions not only enhance our understanding of intracellular biological mechanisms but also hold significant importance for drug research and development, as well as disease treatment. The KeyTec® TR-FRET Toolbox series offers a straightforward and rapid means of detecting protein-protein interactions, making it an excellent choice for drug screening and research into drug action mechanisms.
KeyTec® TR-FRET mAb anti-HIS-Green is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HIS-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-GST-Green is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-GST-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-FLAG-Green is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Bright Eu is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the Donor (KeyTec® TR-FRET mAb anti-HIS-Bright Eu*1), and the other protein is labeled (directly or indirectly) with the Acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
The KeyTec® TR - FRET Tool - Box series of products provide flexible solutions for developing protein - protein interaction experiments. This series includes tag antibodies (such as Anti - HIS, Anti - GST, etc.), secondary antibodies (such as Anti - Human IgG, etc.) and proteins (Streptavidin, Protein A, etc.) conjugated with TR-FRET fluorescence resonance energy transfer donors (Solar Eu/Solar Tb) or fluorescence resonance energy transfer acceptors (LA/HX), allowing you to develop your own TR-FRET experiments like 'building blocks'.
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