VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
KeyTec® TR-FRET PD1/PD-L1 Binding Assay Kit is designed for the quantitative measurement of the interaction between human PD1 and PD-L1 proteins. This kit provides a Tag1-PD-L1 protein, a Tag2-PD1 protein, an anti-tag1 antibody labeled with KeyTec® TR-FRET Solar Eu* 1 (mAb anti-Tag1 - Solar Eu) ,an anti-tag2 antibody labeled with KeyTec® TR-FRET LA* 2 (mAb anti-Tag2 - LA) and other detection reagents. These components enable the high-throughput screening of small molecule inhibitors, peptides, or antibody blockers.
KeyTec® TR-FRET cAMP Higher Detection kit can be used to measure cAMP levels in cells. This kit is a competitive immunoassay developed using KeyTec® TR-FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility.
This kit measures serine/threonine kinase (STK) activity, validated for over 150 STKs. It supplies three biotinylated substrates (S1/S2/S3), streptavidin‑HX, and a phospho‑STK antibody labeled with Solar Eu, enabling quantification via phosphorylated substrate detection.
This kit provides a robust TR-FRET-based assay for rapidly identifying blockers of the IL6/IL6R protein–protein interaction. It uses fluorescent donor–acceptor pairs tagged to IL6 and IL6R, respectively; binding brings the tags close enough to generate a FRET signal, while inhibitors reduce this signal, enabling straightforward quantification and high-throughput screening.
KeyTec® TR-FRET mAb anti-HIS-LA is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HIS- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET human cereblon binding kit is designed for the binding analysis of cereblon ligands/inhibitors to wild-type human cereblon.
The KeyTec® TR‑FRET STK S1 Kinase Kit quantifies serine/threonine kinase activity, validated for >50 STKs. It supplies a biotinylated substrate, streptavidin‑HX, and a Solar Eu‑labeled phospho‑STK antibody. The TR‑FRET‑based sandwich assay enables simple, rapid, and sensitive quantification via phosphorylated substrate detection.
This kit offers a TR-FRET-based platform for rapid, high-throughput screening of blockers targeting the IL12/IL12R-β1 protein–protein interaction. The assay relies on fluorescent donor–acceptor pairs conjugated to IL12 and IL12R-β1, respectively; binding brings the pair into proximity to generate a FRET signal, while inhibitory compounds reduce this signal.
KeyTec® TR-FRET human von Hippel−Lindau(VHL) binding kit is designed for the binding analysis of VHL ligands/inhibitors to wild-type human VHL. This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
The KeyTec® TR‑FRET STK S2 Kinase Kit quantifies serine/threonine kinase activity, validated for >30 STKs. It supplies a biotinylated substrate, streptavidin‑HX, and a Solar Eu‑labeled phospho‑STK antibody. The TR‑FRET‑based sandwich assay enables simple, rapid, and sensitive quantification via phosphorylated substrate detection.
This kit provides a TR-FRET-based assay for rapid, high-throughput screening of blockers targeting the IL23/IL23R protein–protein interaction. The assay employs fluorescent donor–acceptor pairs conjugated to IL23 and IL23R, respectively; binding brings the pair into close proximity to generate a FRET signal, while inhibitory compounds reduce this signal.
KeyTec® TR-FRET mAb anti-GST-Solar Eu is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-GST-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
English
