VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
KeyTec® TR-FRET PD1/PD-L1 Binding Assay Kit is designed for the quantitative measurement of the interaction between human PD1 and PD-L1 proteins. This kit provides a Tag1-PD-L1 protein, a Tag2-PD1 protein, an anti-tag1 antibody labeled with KeyTec® TR-FRET Solar Eu* 1 (mAb anti-Tag1 - Solar Eu) ,an anti-tag2 antibody labeled with KeyTec® TR-FRET LA* 2 (mAb anti-Tag2 - LA) and other detection reagents. These components enable the high-throughput screening of small molecule inhibitors, peptides, or antibody blockers.
KeyTec® TR-FRET TKs kinase kit is designed for measuring the Tyrosine kinase (TKs) activity. Tyrosine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 60 tyrosine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho Tyr-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
KeyTec® TR-FRET mAb anti-HIS-LA is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HIS- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET human cereblon binding kit is designed for the binding analysis of cereblon ligands/inhibitors to wild-type human cereblon. This kit provides a tag1- cereblon/DNA damage-binding protein 1 complex ( Tag1-CRBN/DDB1 ), a Thalidomide-HX*1 (a cereblon inhibitor), an anti-tag1 antibody labeled with KeyTec® TR-FRET Solar Eu*2 (mAb anti-Tag1 - Solar Eu) and other detection reagents. These components enable the high-throughput screening of cereblon ligands/inhibitors.
This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
KeyTec® TR-FRET TKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.
KeyTec® TR-FRET BTKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.
KeyTec® TR-FRET mAb anti-GST-Solar Eu is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-GST-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET JAKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.
KeyTec® TR-FRET FGFRs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit（Cat. # A1080001L). For substrate optimization data, please refer to 《KeyTec® TR-FRET Kinase assay platform and assay development guide》 （For more details, please visit our official website or contact our technical support team）.
KeyTec® TR-FRET mAb anti-GST-Solar Tb is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-GST-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET human von Hippel−Lindau(VHL) binding kit is designed for the binding analysis of VHL ligands/inhibitors to wild-type human VHL. This kit provides a tag1- VHL/Elongin C/Elongin B protein complex ( Tag1-VCB complex ), a VHL ligand-LA*1 (CAS#1948273-02-6), an anti-tag1 antibody labeled with KeyTec® TR-FRET Solar Eu*2 (mAb anti-Tag1 - Solar Eu) and other detection reagents. These components enable the high-throughput screening of VHL ligands/inhibitors. This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
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