TR FRET Assay Kit

The KeyTec® TR-FRET Hybridoma screening kit (His antigen, Rat IgG) is designed for the simple and rapid screening of positive rat hybridoma clones based on antigen-antibody affinity in cell supernatant.

KeyTec® TR-FRET Human STING WT Protein Binding Assay Kit can be used to measure the binding of ligands or inhibitors to wild-type human STING protein. This kit is a competitive immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high specificity, high sensitivity, and excellent reproducibility.

KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between wild-type BTK protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.

KeyTec® TR-FRET mAb anti-GST-LA is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-GST-LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

The KeyTec^® TR‑FRET CDKs Kinase Kit quantifies CDK activity via phosphorylation of Rb S780. It includes a LA‑labeled substrate and a Solar Eu‑labeled phospho‑antibody. The TR‑FRET sandwich assay provides simple, rapid, and sensitive quantification.

KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between BRD4 protein and the VHL/Elongin C/Elongin B protein complex (VHL/Elongin C/Elongin B protein complex, hereinafter referred to as the VCB complex).

KeyTec® TR-FRET mAb anti-GST-HX is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-GST-HX*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

The KeyTec^® TR‑FRET CDKs Kinase Kit detects activity of CDK2 and CDK5, which phosphorylate ATF‑2 at T71. It supplies a LA‑labeled substrate and a Solar Eu‑labeled phospho‑antibody. The TR‑FRET sandwich assay involves kinase reaction (substrate phosphorylation) and detection (antibody binding to phosphorylated site), generating a signal proportional to activity. The assay is simple, rapid, sensitive, and reproducible.

KeyTec® TR-FRET Human FcRn Binding Kit is designed to screen IgGs and FcRn inhibitors that bind to Human FcRn, and provides the ideal solution to assess the half-life of different IgGs. This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec® TR-FRET TKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.

KeyTec^® TR-FRET BTKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.

KeyTec® TR-FRET mAb anti-FLAG-Solar Eu is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-FLAG-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

KeyTec® TR-FRET Human CD16a (FcγRIIIA, 176V) Binding Kit is designed to screen IgGs that bind to Human CD16a (FcγRⅢA, 176V), and provides the ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD16a (FcγRIIIA, 176V) is hereinafter referred as CD16a (176V). This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec^® TR-FRET JAKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.