TR FRET Assay Kit

The KeyTec® TR-FRET Hybridoma screening kit (His antigen, Rat IgG) is designed for the simple and rapid screening of positive rat hybridoma clones based on antigen-antibody affinity in cell supernatant.

KeyTec® TR-FRET Human PARG Inhibitor Screening Kit is designed for screening inhibitors of the PARG enzyme. This kit employs an immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.

The KeyTec^® TR‑FRET STK S3 Kinase Kit quantifies serine/threonine kinase activity, validated for >50 STKs. It supplies a biotinylated substrate, streptavidin‑HX, and a Solar Eu‑labeled phospho‑STK antibody. The TR‑FRET‑based sandwich assay enables simple, rapid, and sensitive quantification via phosphorylated substrate detection.

This kit offers a TR-FRET-based assay for rapid, high-throughput screening of blockers targeting the IL23/IL12R-β1 protein–protein interaction. The assay uses fluorescent donor–acceptor pairs conjugated to IL23 and IL12R-β1, respectively; binding brings the pair into proximity to generate a FRET signal, while inhibitory compounds reduce this signal.

KeyTec® TR-FRET mAb anti-GST-Solar Tb is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-GST-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520nm, proportional to the extent of protein interaction.

KeyTec® TR-FRET Human FcRn Binding Kit is designed to screen IgGs and FcRn inhibitors that bind to Human FcRn, and provides the ideal solution to assess the half-life of different IgGs. This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between STAT6 protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.

The KeyTec® TR‑FRET Human IL‑17AF/IL‑17RA Binding Assay Kit provides a rapid, accurate, and homogeneous method for high‑throughput screening of blockers of the IL‑17AF–IL‑17RA interaction. This format is ideal for evaluating small molecules, peptides, and antibodies as potential IL‑17 pathway modulators.

KeyTec® TR-FRET Human CD16a (FcγRIIIA, 176V) Binding Kit is designed to screen IgGs that bind to Human CD16a (FcγRⅢA, 176V), and provides the ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD16a (FcγRIIIA, 176V) is hereinafter referred as CD16a (176V). This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec® TR-FRET Human STING WT Protein Binding Assay Kit can be used to measure the binding of ligands or inhibitors to wild-type human STING protein. This kit is a competitive immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high specificity, high sensitivity, and excellent reproducibility.

KeyTec® TR-FRET Human CD16a (FcγRIIIA, 176F) Binding Kit is designed to screen IgGs that bind to Human CD16a (FcγRⅢA, 176F), and provides the ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD16a (FcγRIIIA, 176F) is hereinafter referred as CD16a (176F). This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process. 

KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between wild-type BTK protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.

KeyTec® TR-FRET mAb anti-GST-LA is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-GST-LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.