VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
KeyTec® TR-FRET mAb anti-FLAG-Solar Eu is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-FLAG-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET JAKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.
This kit provides a homogeneous competitive TR-FRET assay for quantifying human IgG levels in various sample types. Using a donor-labeled anti-hFc antibody and an acceptor-labeled Human IgG tracer, the assay measures the competitive displacement signal at 665 nm, where the signal inversely correlates with the target concentration. It offers a rapid, accurate, and broad-range detection method for human IgG quantification.
The KeyTec® TR‑FRET FGFRs substrate‑biotin kinase substrate can be used to develop kinase activity assays. When combined with the KeyTec® TR‑FRET TKs kinase kit (Cat. # A1080001L), it enables the construction of a TR‑FRET activity assay for evaluating relative enzyme activity or inhibitor screening.
The KeyTec® ActRIIA Kinase Kit detects ActRIIA activity by quantifying ADP produced from substrate phosphorylation. The assay includes a kinase reaction and an ADP detection step, enabling rapid kinase activity assessment
KeyTec® TR-FRET mAb anti-FLAG-Solar Tb is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-FLAG-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520nm, proportional to the extent of protein interaction.
The KeyTec® ActRIIB Kinase Kit detects relative ActRIIB (ACVR2B) activity by quantifying ADP generated from a kinase–substrate reaction.
KeyTec® TR-FRET mAb anti-FLAG-LA is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-FLAG-HX is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG- HX*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-MYC-Solar Eu is designed for developing the TR-FRET Assay. The anti-MYC antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one MYC-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-MYC-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-MYC-LA is designed for developing the TR-FRET Assay. The anti-MYC antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one MYC-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-MYC- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-HA-Solar Eu is designed for developing the TR-FRET Assay. The anti-HA antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HA-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HA-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-HA-LA is designed for developing the TR-FRET Assay. The anti-HA antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HA-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HA- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-Solar Eu is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the donor (KeyTec® TR-FRET Streptavidin-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-Solar Tb is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the donor (KeyTec® TR-FRET Streptavidin-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-LA is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the acceptor (KeyTec® TR-FRET Streptavidin- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
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