VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
KeyTec® TR-FRET pAb anti-Rat IgG-LA is designed for developing the TR-FRET Assay. The anti-Rat IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rat IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Rat IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET Protein A-Solar Eu is designed for developing the TR-FRET Assay. Protein A has high affinity for Ig types from different species, particularly IgG. In the Antigen-Antibody Interaction assay, the IgG antibody binds to the donor (KeyTec® TR-FRET Protein A-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET Protein A-LA is designed for developing the TR-FRET Assay. Protein A exhibits high affinity towards various species of Ig, particularly IgG. In the Antigen-Antibody Interaction assay, the IgG antibody binds to the acceptor (KeyTec® TR-FRET Protein A-LA*2), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu*1). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET mAb anti-HIS-Green is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HIS-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-GST-Green is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-GST-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-FLAG-Green is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Bright Eu is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the Donor (KeyTec® TR-FRET mAb anti-HIS-Bright Eu*1), and the other protein is labeled (directly or indirectly) with the Acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
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